en
Scientific article
English

Simultaneous selected reaction monitoring, MS/MS and MS3 quantitation for the analysis of pharmaceutical compounds in human plasma using chip-based infusion

Published inRCM. Rapid communications in mass spectrometry, vol. 18, no. 17, p. 1995-2000
Publication date2004
Abstract

An assay method with mass spectrometric detection was developed for the quantitative analysis of a pharmaceutical compound and its major metabolite in human plasma using chip-based infusion. Liquid-liquid extraction sample preparation was found to be essential to minimize matrix suppression and to achieve a limit of quantitation (LOQ) of 2.5 ng/mL using a 100 microL plasma aliquot. The potential for simultaneous quantitation in selected reaction monitoring (SRM), tandem mass spectrometry (MS/MS) (enhanced product ion), and MS(3) was investigated and found to be very beneficial in improving assay selectivity. A novel concept for monitoring quantitative assay performance using a SRM/MS(3) ratio is proposed.

Keywords
  • Mass Spectrometry/instrumentation/methods
  • Nanotechnology/methods
  • Pharmaceutical Preparations/blood
  • Reproducibility of Results
  • Sensitivity and Specificity
Citation (ISO format)
LEUTHOLD, Luc Alexis et al. Simultaneous selected reaction monitoring, MS/MS and MS3 quantitation for the analysis of pharmaceutical compounds in human plasma using chip-based infusion. In: RCM. Rapid communications in mass spectrometry, 2004, vol. 18, n° 17, p. 1995–2000. doi: 10.1002/rcm.1587
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Article (Accepted version)
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Identifiers
ISSN of the journal0951-4198
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Creation2009/07/21 09:39:00
First validation2009/07/21 09:39:00
Update time2023/03/14 15:10:13
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