UNIGE document Scientific Article
previous document  unige:2372  next document
add to browser collection
Title

Simultaneous selected reaction monitoring, MS/MS and MS3 quantitation for the analysis of pharmaceutical compounds in human plasma using chip-based infusion

Authors
Leuthold, Luc Alexis
Allen, Mark
Baumert, Mark
Published in Rapid Communications in Mass Spectrometry. 2004, vol. 18, no. 17, p. 1995-2000
Abstract An assay method with mass spectrometric detection was developed for the quantitative analysis of a pharmaceutical compound and its major metabolite in human plasma using chip-based infusion. Liquid-liquid extraction sample preparation was found to be essential to minimize matrix suppression and to achieve a limit of quantitation (LOQ) of 2.5 ng/mL using a 100 microL plasma aliquot. The potential for simultaneous quantitation in selected reaction monitoring (SRM), tandem mass spectrometry (MS/MS) (enhanced product ion), and MS(3) was investigated and found to be very beneficial in improving assay selectivity. A novel concept for monitoring quantitative assay performance using a SRM/MS(3) ratio is proposed.
Keywords Mass Spectrometry/instrumentation/methodsNanotechnology/methodsPharmaceutical Preparations/bloodReproducibility of ResultsSensitivity and Specificity
Identifiers
PMID: 15329867
Full text
Structures
Citation
(ISO format)
LEUTHOLD, Luc Alexis et al. Simultaneous selected reaction monitoring, MS/MS and MS3 quantitation for the analysis of pharmaceutical compounds in human plasma using chip-based infusion. In: Rapid Communications in Mass Spectrometry, 2004, vol. 18, n° 17, p. 1995-2000. https://archive-ouverte.unige.ch/unige:2372

118 hits

0 download

Update

Deposited on : 2009-08-10

Export document
Format :
Citation style :