Scientific article
English

Determination of carnitine and acylcarnitines in urine by high-performance liquid chromatography-electrospray ionization ion trap tandem mass spectrometry

Published inJournal of chromatography, vol. 984, no. 2, p. 203-213
Publication date2003
Abstract

A high-performance liquid chromatography-mass spectrometry method has been developed for the simultaneous determination of native carnitine and eight acylcarnitines in urine. The procedure uses a solid-phase extraction on a cation-exchange column and the separation is performed without derivatization within 17 min on a reversed-phase C8 column in the presence of a volatile ion-pairing reagent. The detector was an ion trap mass spectrometer and quantification was carried out in the MS-MS mode. Validation was done for aqueous standards at ranges between 0.75 and 200 micromol/l, depending on the compound. Carnitine was quantified in urine and comparison with a radioenzymatic assay gave a satisfactory correlation (R2 = 0.981). The assay could be successfully applied to the diagnostic of pathological acylcarnitines profile of metabolic disorders in urines of patients suffering from different organic acidurias.

Keywords
  • Carnitine/analogs & derivatives/urine
  • Chromatography, High Pressure Liquid/methods
  • Reproducibility of Results
  • Spectrometry, Mass, Electrospray Ionization/methods
Citation (ISO format)
VERNEZ, Laurence et al. Determination of carnitine and acylcarnitines in urine by high-performance liquid chromatography-electrospray ionization ion trap tandem mass spectrometry. In: Journal of chromatography, 2003, vol. 984, n° 2, p. 203–213. doi: 10.1016/S0021-9673(02)01838-1
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Article (Accepted version)
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Identifiers
Journal ISSN0021-9673
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Technical informations

Creation21/07/2009 13:20:00
First validation21/07/2009 13:20:00
Update time14/03/2023 16:10:11
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