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Title

Determination of carnitine and acylcarnitines in urine by high-performance liquid chromatography-electrospray ionization ion trap tandem mass spectrometry

Authors
Vernez, Laurence
Wenk, Markus
Krähenbühl, Stephan
Published in Journal of Chromatography. A. 2003, vol. 984, no. 2, p. 203-213
Abstract A high-performance liquid chromatography-mass spectrometry method has been developed for the simultaneous determination of native carnitine and eight acylcarnitines in urine. The procedure uses a solid-phase extraction on a cation-exchange column and the separation is performed without derivatization within 17 min on a reversed-phase C8 column in the presence of a volatile ion-pairing reagent. The detector was an ion trap mass spectrometer and quantification was carried out in the MS-MS mode. Validation was done for aqueous standards at ranges between 0.75 and 200 micromol/l, depending on the compound. Carnitine was quantified in urine and comparison with a radioenzymatic assay gave a satisfactory correlation (R2 = 0.981). The assay could be successfully applied to the diagnostic of pathological acylcarnitines profile of metabolic disorders in urines of patients suffering from different organic acidurias.
Keywords Carnitine/analogs & derivatives/urineChromatography, High Pressure Liquid/methodsReproducibility of ResultsSpectrometry, Mass, Electrospray Ionization/methods
Identifiers
PMID: 12564691
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VERNEZ, Laurence et al. Determination of carnitine and acylcarnitines in urine by high-performance liquid chromatography-electrospray ionization ion trap tandem mass spectrometry. In: Journal of Chromatography. A, 2003, vol. 984, n° 2, p. 203-213. https://archive-ouverte.unige.ch/unige:2364

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Deposited on : 2009-08-10

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