Ion spray mass spectrometric detection for liquid chromatography: A concentration- or a mass-flow-sensitive device?

Hopfgartner, Gerard; Bean, Karen; Henion, Jack; Henry, Richard

doi:10.1016/0021-9673(93)83323-K

Scientific article

English

Ion spray mass spectrometric detection for liquid chromatography: A concentration- or a mass-flow-sensitive device?

Contributors

Hopfgartner, Gerard

; Bean, Karen; Henion, Jack; Henry, Richard

Published in

Journal of chromatography, vol. 647, no. 1, p. 51-61

Publication date

1993

Abstract

As the mass spectrometer becomes more accepted as a detector for HPLC, its characteristics should become better understood by those performing routine LC-MS experiments. In particular, the ion current response for quantitative analysis studies involving significant dynamic range in concentration for target analytes must be determined as well as other factors that affect MS response. This work describes the concentration-sensitive response for the ion spray (pneumatically-assisted electrospray) LC-MS interface from the chromatographer's perspective. A comparison of LC-MS ion current response in the isocratic mode resulting from studies of a synthetic mixture containing alkyl benzoates is presented. LC-MS total ion current chromatograms from three different column sizes (1 mm I.D., 2.1 mm I.D. and 4.6 mm I.D.) with and without a post-column split, and high-flow ion spray LC-MS without a post-column split illustrates that the former behaves as a concentration-sensitive detector whereas the latter behaves as a mass-flow-sensitive detector. The flexibility of ion spray to high-flow applications allows the use of HPLC eluent flow ranging from 0.001-2.0 ml/min. The use of solvent—buffer post-column addition also allows optimization for improved analyte ion current response.

UNIGE affiliation entities

Faculté des sciences / Section des sciences pharmaceutiques

Citation (ISO format)

HOPFGARTNER, Gerard et al. Ion spray mass spectrometric detection for liquid chromatography: A concentration- or a mass-flow-sensitive device? In: Journal of chromatography, 1993, vol. 647, n° 1, p. 51–61. doi: 10.1016/0021-9673(93)83323-K

Article (Accepted version)

Identifiers

PID : unige:2346
DOI : 10.1016/0021-9673(93)83323-K

Journal ISSN

0021-9673

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06/08/2009 15:33:00

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06/08/2009 15:33:00

Update

14/03/2023 15:10:06

Status update

14/03/2023 15:10:06

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29/10/2024 11:51:22

Ion spray mass spectrometric detection for liquid chromatography: A concentration- or a mass-flow-sensitive device?

Contributors

Published in

Publication date

Abstract

UNIGE affiliation entities

Citation (ISO format)

Main files (1)

Identifiers

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation