en
Scientific article
English

Development and validation of a HPAE-PAD method for the quantification of CGP69669A, a sialyl Lewisx mimetic, in skin permeation studies

Published inBMC. Biomedical chromatography, vol. 26, no. 4, p. 507-511
Publication date2012
Abstract

A simple, rapid, precise and specific isocratic HPAE-PAD method for quantification of CGP69669A was developed and validated. CGP69669A is a glycomimetic of sialyl Lewis(x) and an antagonist of E-selectin with potential application in the treatment of inflammatory skin disease. Quantification was performed using a Dionex CarboPac(TM) PA-200 anion-exchange column (3 × 250 mm) with 100 mm NaOH solution as mobile phase, a flow rate of 0.50 mL/min and an injection volume of 10 μL. A quadruple potential waveform was used to detect the carbohydrate (+0.1 V from 0.00 to 0.40 s, -2.0 V from 0.41 to 0.42 s, +0.6 V at 0.43 s and -0.1 V from 0.44 to 0.50 s with current integrated between 0.20 and 0.40 s for detection) and rafinose was employed as an internal standard. The optimized conditions enabled rapid elution of CGP69669A (at 3.0 min) without interference from solvent peaks or substances present in the skin. The method showed good intra- and inter-day precision and accuracy and the response was linear from 1.0 to 25 µg/mL. This is the first validated direct method for the quantification of CGP69669A. It will now be employed in studies investigating the topical and transdermal delivery of CGP69669A in vitro and in vivo and it should also be of use for other applications of this molecule.

Keywords
  • Animals
  • Chromatography, High Pressure Liquid/economics/methods
  • Chromatography, Ion Exchange/economics/methods
  • E-Selectin/metabolism
  • Limit of Detection
  • Linear Models
  • Oligosaccharides/analysis/pharmacokinetics
  • Skin/metabolism
  • Skin Absorption
  • Swine
Citation (ISO format)
GRATIERI, Tais et al. Development and validation of a HPAE-PAD method for the quantification of CGP69669A, a sialyl Lewis<sup>x</sup> mimetic, in skin permeation studies. In: BMC. Biomedical chromatography, 2012, vol. 26, n° 4, p. 507–511. doi: 10.1002/bmc.1695
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ISSN of the journal0269-3879
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Technical informations

Creation09/05/2012 12:44:00 PM
First validation09/05/2012 12:44:00 PM
Update time03/14/2023 5:40:33 PM
Status update03/14/2023 5:40:33 PM
Last indexation05/02/2024 12:38:25 PM
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