Scientific article

Development and validation of a rapid high-performance liquid chromatography method for the quantification of exenatide

Published inBMC. Biomedical chromatography, vol. 25, no. 7, p. 838-842
Publication date2011

The objective was to develop a simple HPLC method to quantify exenatide--a 39 amino acid residue incretin mimetic used in diabetes therapy. To date, only non-validated, sometimes incomplete, gradient methods have been reported in the literature. Isocratic separation was achieved using a C₄ column and a mixed solvent system, A-B-C (48:45:7, v/v/v; pH* 5.2), where A represents KH₂PO₄ (pH 4.5; 0.1 M) and MeCN (60:40, v/v), B corresponds to NaClO₄ ·H₂O (pH 6.0; 0.2 M) and MeCN (60:40, v/v), and C is water. Exenatide eluted at 3.64 min and the total run time was 6 min. The method was specific and the response was accurate, precise and linear from 0.75 to 25 µg/mL. It was used to quantify exenatide transport across intact and laser-porated porcine skin in vitro as a function of laser fluence [0 (i.e. intact skin), 9 and 15 J/cm², respectively]. Although no permeation was observed using intact skin, cumulative exenatide permeation after 8 h through laser porated skin was 9.6 ± 6.5 and 12.4 ± 6.4 µg/cm² at fluences of 9 and 15 J/cm², respectively. This is the first validated isocratic method for exenatide quantification and it may be of use in quality control analysis and with other biological matrices.

  • Administration, Cutaneous
  • Analysis of Variance
  • Animals
  • Chromatography, High Pressure Liquid/methods
  • Lasers
  • Least-Squares Analysis
  • Peptides/administration & dosage/analysis/pharmacokinetics
  • Permeability
  • Porosity
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Skin/chemistry
  • Swine
  • Venoms/administration & dosage/analysis/pharmacokinetics
Citation (ISO format)
BACHHAV, Yogeshwar, KALIA, Yogeshvar. Development and validation of a rapid high-performance liquid chromatography method for the quantification of exenatide. In: BMC. Biomedical chromatography, 2011, vol. 25, n° 7, p. 838–842. doi: 10.1002/bmc.1526
Main files (1)
Article (Published version)
ISSN of the journal0269-3879

Technical informations

Creation09/03/2012 1:57:00 PM
First validation09/03/2012 1:57:00 PM
Update time03/14/2023 5:40:30 PM
Status update03/14/2023 5:40:30 PM
Last indexation10/18/2023 2:35:53 PM
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