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Title

Pig ear skin ex vivo as a model for in vivo dermatopharmacokinetic studies in man

Authors
Hadgraft, Jonathan
Published in Pharmaceutical Research. 2006, vol. 23, no. 8, p. 1850-6
Abstract OBJECTIVE: The objective was to investigate pig ear skin as a surrogate for human skin in the assessment of topical drug bioavailability by sequential tape-stripping of the stratum corneum (SC). The potential benefits of ex vivo investigations are manifold: ethical approval is not required, multiple replicate experiments are more easily performed, and toxic compounds can be evaluated. MATERIALS AND METHODS: Ex vivo experiments on isolated pig ears were compared with in vivo studies in human volunteers. Four formulations, comprising the model drug, ibuprofen, in different propylene glycol (PG)-water mixtures (25:75, 50:50, 75:25 and 100:0), were compared. RESULTS: Derived dermatopharmacokinetic parameters characterizing the diffusion and partitioning of the drug in the SC ex vivo were consistent with those in vivo following a 30-minute application period. Further, the non-steady-state ex vivo results could be used to predict the in vivo concentration profile of the drug across the SC when a formulation was administered for 3 h (i.e., close to steady-state). CONCLUSIONS: Taken together, the results obtained suggest that pig ear skin ex vivo has promise as a tool for topical formulation evaluation and optimization.
Keywords AdultAlgorithmsAnimalsAnti-Inflammatory AgentsNon-Steroidal/administration & dosage/pharmacokineticsArea Under CurveBiological AvailabilityChromatographyHigh Pressure LiquidData InterpretationStatisticalDiffusionDiffusion ChambersCultureEarExternal/metabolismFemaleHumansIbuprofen/administration & dosage/pharmacokineticsKineticsMaleMiddle AgedPropylene Glycols/chemistrySkin Absorption/physiologySwine
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PMID: 16841197
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HERKENNE, Christophe et al. Pig ear skin ex vivo as a model for in vivo dermatopharmacokinetic studies in man. In: Pharmaceutical Research, 2006, vol. 23, n° 8, p. 1850-6. https://archive-ouverte.unige.ch/unige:22652

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Deposited on : 2012-08-29

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