en
Scientific article
Open access
English

Pig ear skin ex vivo as a model for in vivo dermatopharmacokinetic studies in man

Published inPharmaceutical research, vol. 23, no. 8, p. 1850-1856
Collection
  • Open Access - Licence nationale Springer
Publication date2006
Abstract

OBJECTIVE: The objective was to investigate pig ear skin as a surrogate for human skin in the assessment of topical drug bioavailability by sequential tape-stripping of the stratum corneum (SC). The potential benefits of ex vivo investigations are manifold: ethical approval is not required, multiple replicate experiments are more easily performed, and toxic compounds can be evaluated. MATERIALS AND METHODS: Ex vivo experiments on isolated pig ears were compared with in vivo studies in human volunteers. Four formulations, comprising the model drug, ibuprofen, in different propylene glycol (PG)-water mixtures (25:75, 50:50, 75:25 and 100:0), were compared. RESULTS: Derived dermatopharmacokinetic parameters characterizing the diffusion and partitioning of the drug in the SC ex vivo were consistent with those in vivo following a 30-minute application period. Further, the non-steady-state ex vivo results could be used to predict the in vivo concentration profile of the drug across the SC when a formulation was administered for 3 h (i.e., close to steady-state). CONCLUSIONS: Taken together, the results obtained suggest that pig ear skin ex vivo has promise as a tool for topical formulation evaluation and optimization.

Keywords
  • Adult
  • Algorithms
  • Animals
  • Anti-Inflammatory Agents
  • Non-Steroidal/administration & dosage/pharmacokinetics
  • Area Under Curve
  • Biological Availability
  • Chromatography
  • High Pressure Liquid
  • Data Interpretation
  • Statistical
  • Diffusion
  • Diffusion Chambers
  • Culture
  • Ear
  • External/metabolism
  • Female
  • Humans
  • Ibuprofen/administration & dosage/pharmacokinetics
  • Kinetics
  • Male
  • Middle Aged
  • Propylene Glycols/chemistry
  • Skin Absorption/physiology
  • Swine
Citation (ISO format)
HERKENNE, Christophe et al. Pig ear skin ex vivo as a model for in vivo dermatopharmacokinetic studies in man. In: Pharmaceutical research, 2006, vol. 23, n° 8, p. 1850–1856. doi: 10.1007/s11095-006-9011-8
Main files (1)
Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal0724-8741
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361downloads

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