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Title

Microneme protein 8 - a new essential invasion factor in Toxoplasma gondii

Authors
Kessler, Henning
Herm-Götz, Angelika
Hegge, Stephan
Rauch, Manuel
Frischknecht, Friedrich
Meissner, Markus
Published in Journal of Cell Science. 2008, vol. 121, no. Pt 7, p. 947-56
Abstract Apicomplexan parasites rely on sequential secretion of specialised secretory organelles for the invasion of the host cell. First, micronemes release their content upon contact with the host cell. Second, rhoptries are discharged, leading to the formation of a tight interaction (moving junction) with the host cell, through which the parasite invades. The functional characterisation of several micronemal proteins in Toxoplasma gondii suggests the occurrence of a stepwise process. Here, we show that the micronemal protein MIC8 of T. gondii is essential for the parasite to invade the host cell. When MIC8 is not present, a block in invasion is caused by the incapability of the parasite to form a moving junction with the host cell. We furthermore demonstrate that the cytosolic domain is crucial for the function of MIC8 and can not be functionally complemented by any other micronemal protein characterised so far, suggesting that MIC8 represents a novel, functionally distinct invasion factor in this apicomplexan parasite.
Keywords AnimalsCell Adhesion Molecules/genetics/metabolism/physiologyCell LineFluorescent Antibody TechniqueHumansModels, GeneticProtozoan Proteins/genetics/metabolism/physiologyToxoplasma/genetics/growth & development/metabolism
Identifiers
PMID: 18319299
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Structures
Research group Biologie d'un parasite intracellulaire obligatoire (773)
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(ISO format)
KESSLER, Henning et al. Microneme protein 8 - a new essential invasion factor in Toxoplasma gondii. In: Journal of Cell Science, 2008, vol. 121, n° Pt 7, p. 947-56. https://archive-ouverte.unige.ch/unige:2231

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Deposited on : 2009-07-17

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