UNIGE document Doctoral Thesis
previous document  unige:22149  next document
add to browser collection
Title

Identification of circadian pathways by Synthetic TAndem Repeat PROMoter (STAR-PROM) screening

Author
Director
Defense Thèse de doctorat : Univ. Genève, 2012 - Sc. 4456 - 2012/07/27
Abstract In peripheral tissues circadian gene expression can be driven either by local oscillators or by cyclic systemic cues controlled by the master clock in the brain’s suprachiasmatic nucleus. In the latter case, systemic signals can activate immediate early transcription factors (IETFs) and thereby control rhythmic transcription. In order to identify IETFs induced by diurnal blood-borne signals, we developed a novel and unbiased experimental strategy, dubbed Synthetic TAndem Repeat PROMoter (STAR-PROM) screening. This technique relies on the observation that most transcription factor binding sites exist at a relatively high frequency in random DNA sequences. Using STAR-PROM we identified serum response factor (SRF) as an IETF responding to oscillating signaling proteins present in human and rodent sera. Our data suggest that in mouse liver SRF is regulated via dramatic diurnal changes of actin dynamics, leading to the rhythmic translocation of the SRF coactivator Myocardin-related transcription factor–B (MRTF-B) into the nucleus.
Keywords CircadianDiurnal actin dynamicsSRFMRTFDiurnal signaling proteinsCircadian gene expression
Identifiers
URN: urn:nbn:ch:unige-221497
Full text
Thesis (11.2 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Citation
(ISO format)
GERBER, Alan. Identification of circadian pathways by Synthetic TAndem Repeat PROMoter (STAR-PROM) screening. Université de Genève. Thèse, 2012. https://archive-ouverte.unige.ch/unige:22149

462 hits

17 downloads

Update

Deposited on : 2012-08-15

Export document
Format :
Citation style :