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Doctoral thesis
English

CAVIN-3 regulates negative limb protein complexes of the mammalian circadian oscillator

ContributorsSchneider, Kim
Defense date2012-07-11
Abstract

The molecular circadian oscillators in mammals rely on a negative feedback loop involving the transcriptional repressors PER1, PER2, CRY1 and CRY2. Here we report the identification of CAVIN-3 as a novel, cytoplasmic PER2-interacting protein. We found that cellular CAVIN-3 levels directly influenced circadian clock properties. Loss- and gain-of-function of CAVIN-3 thus shortened and lengthened, respectively, the circadian period in fibroblasts. CAVIN-3 had a marked effect on the abundance of PER and CRY proteins, which were increased and decreased upon CAVIN-3 depletion and overexpression, respectively, and probably on the strength of the PER:CRY interaction, as suggested by mammalian two-hybrid assays. A CAVIN-3 variant carrying a mutated binding site for the previously reported interacting protein, Protein Kinase C (PKC) δ, failed to alter circadian period length. The role of CAVIN-3 in the clockwork may thus at least in part be mediated by its interaction with yet uncharacterized protein kinases.

eng
Keywords
  • Cavin-3
  • Caveolin-1
  • Mammalian circadian gene expression
  • Period 2-interacting proteins
  • PER:CRY interaction
  • Period length
Citation (ISO format)
SCHNEIDER, Kim. CAVIN-3 regulates negative limb protein complexes of the mammalian circadian oscillator. 2012. doi: 10.13097/archive-ouverte/unige:22002
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Creation07/20/2012 4:30:00 PM
First validation07/20/2012 4:30:00 PM
Update time03/14/2023 5:39:02 PM
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