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Amphiphilic dynamic NDI and PDI probes: imaging microdomains in giant unilamellar vesicles

Published inOrganic & biomolecular chemistry, vol. 10, no. 30, p. 6087-6093
Publication date2012
Abstract

Dynamic amphiphiles provide access to transmembrane ion transport, differential sensing and cellular uptake. In this report, we introduce dynamic amphiphiles with fluorescent tails. Core-substituted naphthalenediimides (cNDIs) and perylenediimides (cPDIs) are tested. Whereas the latter suffer from poor partitioning, dynamic cNDI amphiphiles are found to be purifiable by RP-HPLC, to partition selectively into liquid-disordered (Ld) microdomains of mixed lipid bilayers and to activate DNA as transporters. Importantly, fluorescence properties, partitioning and activity can be modulated by changes in the structure of mixed amphiphiles. These results confirm the potential of dynamic fluorescent amphiphiles to selectively label extra- and intracellular membrane domains and visualize biological function.

Citation (ISO format)
ALONSO, David et al. Amphiphilic dynamic NDI and PDI probes: imaging microdomains in giant unilamellar vesicles. In: Organic & biomolecular chemistry, 2012, vol. 10, n° 30, p. 6087–6093. doi: 10.1039/c2ob25119a
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ISSN of the journal1477-0520
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Technical informations

Creation07/12/2012 2:01:00 PM
First validation07/12/2012 2:01:00 PM
Update time03/14/2023 5:38:40 PM
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