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Title

In vivo labelling of resting monocytes in the reticuloendothelial system with fluorescent iron oxide nanoparticles prior to injury reveals that they are mobilized to infarcted myocardium

Authors
Petri-Fink, Alke
Hofmann, Heinrich
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Published in European Heart Journal. 2010, vol. 31, no. 11, p. 1410-1420
Abstract AIMS: To evaluate the feasibility of loading resting monocytes/macrophages by intravenous (i.v.) injection of fluorescent iron oxide nanoparticles prior to injury and tracking of these cells in the very same animal to myocardial infarction (MI) by magnetic resonance imaging (MRI) and optical imaging. METHODS AND RESULTS: Rats were injected with fluorescent iron oxide nanoparticles (10 mg/kg) (n = 15) prior to injury. After disappearance of the nanoparticles from the blood, MI was induced. Monocytes/macrophages were then tracked in the very same animal by MRI and optical imaging. Control groups were (i) non-injected animals (n = 15), (ii) injected animals associated with a sham operation (n = 8), and (iii) animals treated with an anti-inflammatory agent (n = 6). The presence of iron-loaded cells can be detected by MRI in vivo in the infarcted myocardium. Here, we showed that the detection of inflammatory cells in vivo correlated well with ex vivo imaging (MRI and reflectance fluorescence) and histology. We also showed that the method is robust enough to depict changes in the inflammatory response. CONCLUSION: This study demonstrates that resting monocytes/macrophages can be loaded in vivo by a simple i.v. injection of fluorescent superparamagnetic iron oxide nanoparticles prior to injury and then tracked, in the same animal, in a model of ischaemia-reperfusion leading to myocardial infarct. Although previous studies of macrophages infiltration following MI have labelled the macrophages after injury, this study, for the first time, has pre-load the resting monocytes with fluorescent iron oxide nanoparticles.
Keywords AnimalsAntigens, CDAntigens, Differentiation, MyelomonocyticCell Movement/*physiologyFerric Compounds/*diagnostic useFluorescenceMacrophages/*metabolism/physiologyMagnetic Resonance ImagingMetal Nanoparticles/*diagnostic useMonocytes/*metabolism/physiologyMononuclear Phagocyte SystemMyocardial Infarction/*diagnosisMyocardial Reperfusion Injury/diagnosisRatsRats, Wistar
Identifiers
PMID: 20023288
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Structures
Research groups Biologie du myocarde (22)
Imagerie et radiologie interventionnelle du foie, des voies biliaires et du pancréas (316)
Imagerie cardiaque fonctionnelle (541)
L'athérosclérose et ses complications cliniques (591)
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MONTET-ABOU, Karin et al. In vivo labelling of resting monocytes in the reticuloendothelial system with fluorescent iron oxide nanoparticles prior to injury reveals that they are mobilized to infarcted myocardium. In: European Heart Journal, 2010, vol. 31, n° 11, p. 1410-1420. https://archive-ouverte.unige.ch/unige:21153

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Deposited on : 2012-05-23

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