UNIGE document Scientific Article
previous document  unige:21151  next document
add to browser collection

Single administration of the CXC chemokine-binding protein Evasin-3 during ischemia prevents myocardial reperfusion injury in mice

Authors show hidden authors show all authors [1 - 10]
Published in Arteriosclerosis, thrombosis, and vascular biology. 2010, vol. 30, no. 7, p. 1371-1377
Abstract OBJECTIVE: Evasins (chemokine-binding proteins) have been shown to selectively neutralize chemokine bioactivity. We investigated the potential benefits of Evasin-3 on mouse myocardial ischemia/reperfusion injury. METHODS AND RESULTS: In vivo and ex vivo (Langendorff model) left coronary artery ligature was performed in C57Bl/6 mice. Coronary occlusion was maintained for 30 minutes, followed by different times (up to 24 hours) of reperfusion. Five minutes after coronary occlusion, mice received 1 intraperitoneal injection of Evasin-3 or vehicle. Infarct size was assessed histologically and by serum cardiac troponin I ELISA. In vitro neutrophil chemotaxis, immunohistology, oxidative stress quantification, real-time RT-PCR analysis of leukocyte chemoattractants, and Western blots for cardioprotective intracellular pathway activation were performed. Evasin-3 reduced infarct size and cardiac troponin I levels compared with vehicle. This effect was associated with the reduction of neutrophil infiltration and reactive oxygen species production within the infarcted myocardium. Evasin-3 did not reduce infarct size in the absence of circulating neutrophils (Langendorff model). Evasin-3 did not influence the activation of intracellular cardioprotective pathways or the expression of leukocyte chemoattractants during early phases of reperfusion. CONCLUSIONS: Single administration of Evasin-3 during myocardial ischemia significantly reduced infarct size by preventing CXC chemokine-induced neutrophil recruitment and reactive oxygen species production in myocardial ischemia/reperfusion.
Keywords AnimalsAnti-Inflammatory Agents/*administration & dosageBiological Markers/bloodBlotting, WesternChemotaxis, Leukocyte/drug effectsDisease Models, AnimalDose-Response Relationship, DrugEnzyme-Linked Immunosorbent AssayImmunohistochemistryInjections, IntraperitonealMaleMiceMice, Inbred C57BLMyocardial Infarction/etiology/immunology/pathology/*prevention & controlMyocardial Ischemia/complications/*drug therapy/immunology/pathologyMyocardial Reperfusion Injury/etiology/immunology/pathology/*prevention & controlMyocardium/*immunology/metabolism/pathologyNeutrophil Infiltration/drug effectsOxidative Stress/drug effectsPerfusionPhosphorylationReceptors, CXCR/*administration & dosageReverse Transcriptase Polymerase Chain ReactionSignal TransductionTroponin I/blood
PMID: 20413731
Full text
Article - document accessible for UNIGE members only Limited access to UNIGE
Research groups Biologie du myocarde (22)
L'athérosclérose et ses complications cliniques (591)
(ISO format)
MONTECUCCO, Fabrizio et al. Single administration of the CXC chemokine-binding protein Evasin-3 during ischemia prevents myocardial reperfusion injury in mice. In: Arteriosclerosis, thrombosis, and vascular biology, 2010, vol. 30, n° 7, p. 1371-1377. doi: 10.1161/ATVBAHA.110.206011 https://archive-ouverte.unige.ch/unige:21151

505 hits

0 download


Deposited on : 2012-05-23

Export document
Format :
Citation style :