Scientific article

Single administration of the CXC chemokine-binding protein Evasin-3 during ischemia prevents myocardial reperfusion injury in mice

Published inArteriosclerosis, thrombosis, and vascular biology, vol. 30, no. 7, p. 1371-1377
Publication date2010

OBJECTIVE: Evasins (chemokine-binding proteins) have been shown to selectively neutralize chemokine bioactivity. We investigated the potential benefits of Evasin-3 on mouse myocardial ischemia/reperfusion injury. METHODS AND RESULTS: In vivo and ex vivo (Langendorff model) left coronary artery ligature was performed in C57Bl/6 mice. Coronary occlusion was maintained for 30 minutes, followed by different times (up to 24 hours) of reperfusion. Five minutes after coronary occlusion, mice received 1 intraperitoneal injection of Evasin-3 or vehicle. Infarct size was assessed histologically and by serum cardiac troponin I ELISA. In vitro neutrophil chemotaxis, immunohistology, oxidative stress quantification, real-time RT-PCR analysis of leukocyte chemoattractants, and Western blots for cardioprotective intracellular pathway activation were performed. Evasin-3 reduced infarct size and cardiac troponin I levels compared with vehicle. This effect was associated with the reduction of neutrophil infiltration and reactive oxygen species production within the infarcted myocardium. Evasin-3 did not reduce infarct size in the absence of circulating neutrophils (Langendorff model). Evasin-3 did not influence the activation of intracellular cardioprotective pathways or the expression of leukocyte chemoattractants during early phases of reperfusion. CONCLUSIONS: Single administration of Evasin-3 during myocardial ischemia significantly reduced infarct size by preventing CXC chemokine-induced neutrophil recruitment and reactive oxygen species production in myocardial ischemia/reperfusion.

  • Animals
  • Anti-Inflammatory Agents/*administration & dosage
  • Biological Markers/blood
  • Blotting, Western
  • Chemotaxis, Leukocyte/drug effects
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Enzyme-Linked Immunosorbent Assay
  • Immunohistochemistry
  • Injections, Intraperitoneal
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Myocardial Infarction/etiology/immunology/pathology/*prevention & control
  • Myocardial Ischemia/complications/*drug therapy/immunology/pathology
  • Myocardial Reperfusion Injury/etiology/immunology/pathology/*prevention & control
  • Myocardium/*immunology/metabolism/pathology
  • Neutrophil Infiltration/drug effects
  • Oxidative Stress/drug effects
  • Perfusion
  • Phosphorylation
  • Receptors, CXCR/*administration & dosage
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction
  • Troponin I/blood
Citation (ISO format)
MONTECUCCO, Fabrizio et al. Single administration of the CXC chemokine-binding protein Evasin-3 during ischemia prevents myocardial reperfusion injury in mice. In: Arteriosclerosis, thrombosis, and vascular biology, 2010, vol. 30, n° 7, p. 1371–1377. doi: 10.1161/ATVBAHA.110.206011
Updates (1)
ISSN of the journal1079-5642

Technical informations

Creation05/23/2012 8:53:38 AM
First validation05/23/2012 8:53:38 AM
Update time03/14/2023 5:34:41 PM
Status update03/14/2023 5:34:41 PM
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