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Evaluation of molecular assays for rapid detection of methicillin-resistant Staphylococcus aureus

Malhotra-Kumar, Surbhi
Van Heirstraeten, Liesbet
Lee, Andie
Abrahantes, Jose Cortinas
Lammens, Christine
Vanhommerig, Evelyn
Molenberghs, Geert
Aerts, Marc
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Published in Journal of Clinical Microbiology. 2010, vol. 48, no. 12, p. 4598-4601
Abstract The diagnostic sensitivities of the BD GeneOhm and Cepheid Xpert assays were compared using culture on log-serial dilutions of well-characterized methicillin-resistant Staphylococcus aureus (MRSA) and non-MRSA strains and on nasal and groin swabs from patients with histories of MRSA carriage. The sensitivities of GeneOhm and Xpert were high at 10(3)-CFU/ml MRSA concentrations (92.3% and 96.3%, respectively) although decreased considerably (<35%) at a 1-log-lower concentration. Unexpectedly, both assays also detected select coagulase-negative staphylococci, which requires further evaluation.
Keywords Bacteriological Techniques/*methodsCarrier State/*diagnosis/microbiologyCoagulase/geneticsGroin/microbiologyHumansMethicillin-Resistant Staphylococcus aureus/*isolation & purificationMolecular Diagnostic Techniques/*methodsNasal Mucosa/microbiologySensitivity and SpecificityStaphylococcal Infections/*diagnosis/microbiology
PMID: 20943869
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Research group Groupe Harbarth Stephan (Staphylocoques dorés résistants à la méthicilline) (866)
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MALHOTRA-KUMAR, Surbhi et al. Evaluation of molecular assays for rapid detection of methicillin-resistant Staphylococcus aureus. In: Journal of Clinical Microbiology, 2010, vol. 48, n° 12, p. 4598-4601. https://archive-ouverte.unige.ch/unige:21107

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Deposited on : 2012-05-23

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