UNIGE document Scientific Article
previous document  unige:21076  next document
add to browser collection
Title

Induction of cortical endoplasmic reticulum by dimerization of a coatomer-binding peptide anchored to endoplasmic reticulum membranes

Authors
Lavieu, Gregory
Shi, Lei
Geiling, Michael
Ravazzola, Mariella
Wieland, Felix
Rothman, James E.
Published in Proceedings of the National Academy of Sciences. 2010, vol. 107, no. 15, p. 6876-6881
Abstract Cortical endoplasmic reticulum (cER) is a permanent feature of yeast cells but occurs transiently in most animal cell types. Ist2p is a transmembrane protein that permanently localizes to the cER in yeast. When Ist2 is expressed in mammalian cells, it induces abundant cER containing Ist2. Ist2 cytoplasmic C-terminal peptide is necessary and sufficient to induce cER. This peptide sequence resembles classic coat protein complex I (COPI) coatomer protein-binding KKXX signals, and indeed the dimerized peptide binds COPI in vitro. Controlled dimerization of this peptide induces cER in cells. RNA interference experiments confirm that coatomer is required for cER induction in vivo, as are microtubules and the microtubule plus-end binding protein EB1. We suggest that Ist2 dimerization triggers coatomer binding and clustering of this protein into domains that traffic at the microtubule growing plus-end to generate the cER beneath the plasma membrane. Sequences similar to the Ist2 lysine-rich tail are found in mammalian STIM proteins that reversibly induce the formation of cER under calcium control.
Keywords AnimalsCOP-Coated Vesicles/*chemistry/metabolismCell Membrane/metabolismCytoplasm/metabolismDimerizationEndoplasmic Reticulum/*metabolismHeLa CellsHumansIntracellular Membranes/*metabolismMicroscopy, Confocal/methodsPeptides/chemistryPlasmids/metabolismProtein BindingRNA InterferenceRats
Identifiers
PMID: 20351264
Full text
Structures
Research group Phagocytose (140)
Citation
(ISO format)
LAVIEU, Gregory et al. Induction of cortical endoplasmic reticulum by dimerization of a coatomer-binding peptide anchored to endoplasmic reticulum membranes. In: Proceedings of the National Academy of Sciences, 2010, vol. 107, n° 15, p. 6876-6881. https://archive-ouverte.unige.ch/unige:21076

252 hits

0 download

Update

Deposited on : 2012-05-23

Export document
Format :
Citation style :