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Title

Analysis of the pancreatic low molecular weight proteome in an animal model of acute pancreatitis
Authors
Lassout, Olivier
Pastor, Catherine
Fetaud-Lapierre, Vanessa
Hochstrasser, Denis
Frossard, Jean-Louis
Lescuyer, Pierre
Published in Journal of Proteome Research. 2010, vol. 9, no. 9, p. 4535-4544
Abstract We used a peptidomic approach for the analysis of the low molecular weight proteome in rat pancreatic tissue extracts. The goal was to develop a method that allows identifying endogenous peptides produced in the pancreas in the course of acute pancreatitis. The workflow combines peptides enrichment by centrifugal ultrafiltration, fractionation by isoelectric focusing, and LC-MS/MS analysis without prior enzymatic digestion. The method was assessed on pancreatic extracts from 3 rats with caerulein-induced pancreatitis and 3 healthy controls. A qualitative analysis of the peptide patterns obtained from the different samples was performed to determine the main biological processes associated to the identified peptides. Comparison of peptidomic and immunoblot data for alpha-tubulin, beta-tubulin and coatomer gamma showed that the correlation between the number of identified peptides and the protein abundance was variable. Nevertheless, peptidomic analysis highlighted inflammatory and stress proteins, which peptide pattern was related to acute pancreatitis pathobiology. For these proteins, the higher number of peptides in pancreatitis samples reflected an increase in protein abundance. Moreover, for murinoglobulin-1 or carboxypeptidase B, peptide pattern could be related to protein function. These data suggest that peptidomic analysis is a complementary approach to proteomics for investigating pathobiological processes involved in acute pancreatitis.
Keywords Acute Disease — Amino Acid Sequence — Animals — Caerulein — Chromatography, Liquid — Disease Models, Animal — Heat-Shock Proteins/chemistry/metabolism — Immunoblotting — Inflammation — Male — Molecular Sequence Data — Molecular Weight — Pancreatitis/chemically induced/*metabolism — Peptides/*chemistry/metabolism — Proteins/chemistry/metabolism — Proteome/*chemistry/metabolism — Proteomics/*methods — Rats — Rats, Sprague-Dawley — Tandem Mass Spectrometry
Identifiers
PMID: 20604530
Full text
Article (Author postprint) (2.6 MB) - public document Free access
Structures
Faculté de médecine / Section de médecine clinique / Département de médecine interne des spécialités
Faculté de médecine / Section de médecine clinique / Département de radiologie et informatique médicale
Faculté de médecine / Section de médecine fondamentale / Département de biologie structurale et bioinformatique
Research groups La protéomique au service de la clinique (270)
Physiopathologie de la pancréatite aiguë (601)
Pharmacologie et imagerie du foie (664)
Citation
(ISO format) 		LASSOUT, Olivier et al. Analysis of the pancreatic low molecular weight proteome in an animal model of acute pancreatitis. In: Journal of Proteome Research, 2010, vol. 9, n° 9, p. 4535-4544. https://archive-ouverte.unige.ch/unige:21074

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Deposited on : 2012-05-23

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