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Scientific article
English

Isolation of nucleoli

Published inCurrent protocols in cell biology, vol. Chapter 3, Unit3 36
Publication date2010
Abstract

Nucleoli are now recognized as multi-functional nuclear domains involved in several fundamental cell processes such as ribosome biogenesis, regulation of the assembly of non-ribosomal ribonucleoprotein complexes, tRNA maturation, sequestration of protein, viral infection, and cellular ageing. Extensive proteomic analyses of these nucleolar domains after their purification have contributed to the description of their multiple biological functions. Because nucleoli are the largest and densest nuclear structures, they are easily amenable to purification from nuclei of cultured animal cells using the protocol described in this unit.

Keywords
  • Animals
  • Cell Culture Techniques/*methods
  • Cell Fractionation/*methods
  • Cell Nucleolus/*chemistry
  • Electrophoresis, Polyacrylamide Gel/*methods
  • Humans
Citation (ISO format)
HACOT, Sabine et al. Isolation of nucleoli. In: Current protocols in cell biology, 2010, vol. Chapter 3, p. Unit3 36. doi: 10.1002/0471143030.cb0336s47
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ISSN of the journal1934-2616
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