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Mitochondrial fission and cristae disruption increase the response of cell models of Huntington's disease to apoptotic stimuli

Hudec, Roman
Lopreiato, Raffaele
Ermak, Gennady
Lim, Dmitri
Malorni, Walter
Davies, Kelvin J. A.
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Published in EMBO Molecular Medicine. 2010, vol. 2, no. 12, p. 490-503
Abstract Huntington's disease (HD), a genetic neurodegenerative disease caused by a polyglutamine expansion in the Huntingtin (Htt) protein, is accompanied by multiple mitochondrial alterations. Here, we show that mitochondrial fragmentation and cristae alterations characterize cellular models of HD and participate in their increased susceptibility to apoptosis. In HD cells, the increased basal activity of the phosphatase calcineurin dephosphorylates the pro-fission dynamin related protein 1 (Drp1), increasing its mitochondrial translocation and activation, and ultimately leading to fragmentation of the organelle. The fragmented HD mitochondria are characterized by cristae alterations that are aggravated by apoptotic stimulation. A genetic analysis indicates that correction of mitochondrial elongation is not sufficient to rescue the increased cytochrome c release and cell death observed in HD cells. Conversely, the increased apoptosis can be corrected by manoeuvres that prevent fission and cristae remodelling. In conclusion, the cristae remodelling of the fragmented HD mitochondria contributes to their hypersensitivity to apoptosis.
Keywords Animals*ApoptosisCell LineCells, CulturedCytochromes c/metabolismFemaleGTP Phosphohydrolases/genetics/metabolismHumansHuntington Disease/genetics/metabolism/*physiopathologyMaleMiceMicroscopy, Electron, TransmissionMicrotubule-Associated Proteins/genetics/metabolismMitochondria/genetics/*physiology/ultrastructureMitochondrial Proteins/genetics/metabolismModels, BiologicalNeurons/cytology/metabolismProtein Transport
PMID: 21069748
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Research group Les mitochondries dans la vie cellulaire (850)
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COSTA, Veronica et al. Mitochondrial fission and cristae disruption increase the response of cell models of Huntington's disease to apoptotic stimuli. In: EMBO Molecular Medicine, 2010, vol. 2, n° 12, p. 490-503. doi: 10.1002/emmm.201000102 https://archive-ouverte.unige.ch/unige:20858

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Deposited on : 2012-05-23

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