UNIGE document Scientific Article
previous document  unige:20783  next document
add to browser collection
Title

A new method to measure local oxygen consumption in human skeletal muscle during dynamic exercise using near-infrared spectroscopy

Authors
Cooper, Chris E.
Wittekind, Anna L.
Beneke, Ralph
Elwell, Clare E.
Leung, Terence S.
Published in Physiological Measurement. 2010, vol. 31, no. 9, p. 1257-1269
Abstract Near infrared spectroscopy (NIRS) can readily report on changes in blood volume and oxygenation. However, it has proved more problematic to measure real-time changes in blood flow and oxygen consumption. Here we report the development of a novel method using NIRS to measure local oxygen consumption in human muscle. The method utilizes the blood volume changes induced by the muscle pump during rhythmically contracting exercising skeletal muscle. We found that the saturation of the blood during the contraction phase was lower than that during the relaxation phase. The calculated oxygen drop was then divided by the contraction time to generate a value for the muscle oxygen consumption in the optical region of interest. As a test we measured the muscle oxygen consumption in the human vastus lateralis during exercise on a cycle ergometer by 11 trained male athletes (32 +/- 11 years old) at 40% and 110% peak aerobic power. We saw an increase from 13.78 micromol 100 g(-1) min(-1) to 19.72 micromol 100 g(-1) min(-1) with the increase in power. The measurements are theoretically exempt from usual NIRS confounders such as myoglobin and adipose tissue and could provide a useful tool for studying human physiology.
Keywords AdultBlood VolumeExercise/*physiologyHemoglobins/metabolismHumansMaleMuscle, Skeletal/*metabolism/physiology*Oxygen ConsumptionOxyhemoglobins/metabolismSpectrophotometry, Infrared/*methodsTime Factors
Identifiers
PMID: 20702918
Full text
Structures
Research groups Groupe Binzoni Tiziano (radiologie) (234)
Traitement d'images médicales (893)
Citation
(ISO format)
BINZONI, Tiziano et al. A new method to measure local oxygen consumption in human skeletal muscle during dynamic exercise using near-infrared spectroscopy. In: Physiological Measurement, 2010, vol. 31, n° 9, p. 1257-1269. https://archive-ouverte.unige.ch/unige:20783

199 hits

0 download

Update

Deposited on : 2012-05-23

Export document
Format :
Citation style :