en
Scientific article
English

Administration of growth hormone in selectively protein-deprived rats decreases BMD and bone strength

Published inBone, vol. 46, no. 6, p. 1574-1581
Publication date2010
Abstract

INTRODUCTION: Isocaloric protein undernutrition is associated with decreased bone mass and decreased bone strength, together with lower IGF-I levels. It remains unclear whether administration of growth hormone (GH) corrects these alterations in bone metabolism. MATERIALS AND METHODS: Six-month-old female rats were fed isocaloric diets containing either 2.5% or 15% casein for 2 weeks. Bovine growth hormone (bGH, 0.5 or 2.5mg/kg of body weight) or vehicle was then administered as subcutaneous injections, twice daily, to rats on either diet for 4 weeks. At the proximal tibia, analysis of bone mineral density (BMD), maximal load and histomorphometry were performed. In addition, urinary deoxypyridinoline, plasma osteocalcin and IGF-I concentrations were measured. Weight was monitored weekly. RESULTS: bGH caused a dose-dependent increase in plasma IGF-I regardless of the dietary protein content. However, bGH dose-dependently decreased BMD and bone strength in rats fed the low-protein diet. There was no significant effect of bGH on BMD in rats fed the normal protein diet within this short-term treatment period, however bone formation as detected by histomorphometry was improved in this group but not the low-protein group. Osteoclast surface was increased in the low-protein bGH-treated animals only. Changes in bone turnover markers were detectable under both normal and low-protein diets. CONCLUSION: These results emphasize the major importance of dietary protein intake in the bone response to short-term GH administration, and highlight the need for further investigation into the effects of GH treatment in patients with reduced protein intake.

Keywords
  • Animals
  • Biomechanics/drug effects
  • Body Weight
  • Bone Density/*drug effects
  • Bone and Bones/*drug effects/metabolism/*physiology
  • Caseins
  • Cattle
  • Diet, Protein-Restricted/adverse effects
  • Female
  • Growth Hormone/*pharmacology
  • Muscle, Skeletal/drug effects
  • Rats
  • Rats, Sprague-Dawley
Citation (ISO format)
AMMANN, Patrick et al. Administration of growth hormone in selectively protein-deprived rats decreases BMD and bone strength. In: Bone, 2010, vol. 46, n° 6, p. 1574–1581. doi: 10.1016/j.bone.2010.02.015
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ISSN of the journal1873-2763
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