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Donor-specific antibody levels and three generations of crossmatches to predict antibody-mediated rejection in kidney transplantation
|Published in||Transplantation. 2010, vol. 90, no. 2, p. 160-167|
|Abstract||BACKGROUND: This study evaluated the prognostic impact of pretransplant donor-specific anti-human leukocyte antigen antibodies (DSA) detected by single-antigen beads and compared the three generations of crossmatch (XM) tests in kidney transplantation. METHODS: Thirty-seven T-cell complement-dependent cytotoxicity crossmatch (CXM) negative living donor kidney recipients with a retrospectively positive antihuman leukocyte antigen antibody screening assay were included. A single-antigen bead test, a flow cytometry XM, and a Luminex XM (LXM) were retrospectively performed, and the results were correlated with the occurrence of antibody-mediated rejections (AMRs) and graft function. RESULTS: We found that (1) pretransplant DSA against class I (DSA-I), but not against class II, are predictive for AMR, resulting in a sensitivity of 75% and a specificity of 90% at a level of 900 mean fluorescence intensity (MFI); (2) with increasing strength of DSA-I, the sensitivity for AMR is decreasing to 50% and the specificity is increasing to 100% at 5200 MFI; (3) the LXM for class I, but not for class II, provides a higher accuracy than the flow cytometry XM and the B-cell CXM. The specificity of all XMs is increased greatly in combination with DSA-I values more than or equal to 900 MFI. CONCLUSIONS: In sensitized recipients, the best prediction of AMR and consecutively reduced graft function is delivered by DSA-I alone at high strength or by DSA-I at low strength in combination with the LXM or CXM.|
|Keywords||Adult — Female — Flow Cytometry — Graft Rejection/blood/ diagnosis/immunology/prevention & control — HLA Antigens/blood/immunology — Histocompatibility Testing/methods — Humans — Immunosuppression/methods — Isoantibodies/ blood — Kidney Transplantation/ immunology — Living Donors — Lymphocytes/immunology — Male — Middle Aged — Prognosis — ROC Curve — Reoperation/statistics & numerical data — Retrospective Studies — Tissue Donors|
|Research group||Transplantation Immunologie et Osteoimmunologie (561)|
|RIETHMULLER, Sebastian et al. Donor-specific antibody levels and three generations of crossmatches to predict antibody-mediated rejection in kidney transplantation. In: Transplantation, 2010, vol. 90, n° 2, p. 160-167. https://archive-ouverte.unige.ch/unige:20700|