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Title

New diagnostic real-time PCR for specific detection of Parachlamydia acanthamoebae DNA in clinical samples

Authors
Casson, Nicola
Greub, Gilbert
Published in Journal of clinical microbiology. 2008, vol. 46, no. 4, p. 1491-3
Abstract Given the low sensitivity of amoebal coculture, we developed a specific real-time PCR for the detection of Parachlamydia. The analytical sensitivity was high, and the inter- and intrarun variabilities were low. When the PCR was applied to nasopharyngeal aspirates, it was positive for six patients with bronchiolitis. Future studies should assess the role of Parachlamydia in bronchiolitis.
Keywords Acanthamoeba/microbiologyAdolescentAnimalsBronchiolitis/diagnosis/microbiologyChild, PreschoolChlamydiales/genetics/isolation & purificationDNA, Bacterial/analysisFemaleGram-Negative Bacterial Infections/diagnosis/microbiologyHumansInfantMalePolymerase Chain Reaction/methodsReproducibility of ResultsSensitivity and Specificity
Identifiers
PMID: 18234873
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Structures
Research groups Etiologie des pneumonies et marqueurs inflammatoires chez l'enfant fébrile (183)
Groupe Posfay-Barbe Clara (pédiatrie générale) (853)
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CASSON, Nicola et al. New diagnostic real-time PCR for specific detection of Parachlamydia acanthamoebae DNA in clinical samples. In: Journal of clinical microbiology, 2008, vol. 46, n° 4, p. 1491-3. https://archive-ouverte.unige.ch/unige:2067

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Deposited on : 2009-06-17

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