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Title

Diagnostic value of anti-cyclic citrullinated peptides and association with HLA-DRB1 shared epitope alleles in African rheumatoid arthritis patients

Authors
Singwe-Ngandeu, Madeleine
Published in Arthritis Research and Therapy. 2010, vol. 12, no. 2, p. R36
Abstract INTRODUCTION: The purpose of this study was to examine the diagnostic performance of autoantibodies against citrullinated peptides/proteins (ACPA) and to determine the prevalence of HLA-DRB1 shared epitope alleles (SE) in African patients with rheumatoid arthritis (RA). METHODS: Serum levels of anti-cyclic citrullinated peptides antibodies (anti-CCP2, anti-CCP3), IgM and IgA rheumatoid factors (RF) were measured by enzyme-linked immunosorbent assay in the serum of 56 consecutive RA patients regularly followed in the Rheumatology Unit of the School of Medicine, University of Yaounde, Yaounde, Cameroon. Genotyping of HLA-DRB1 alleles was performed by polymerase chain reaction and hybridization with sequence-specific oligonucleotide probes on microbeads arrays. Fifty-one patients with other inflammatory rheumatic diseases and 50 healthy individuals were included as controls. RESULTS: An anti-CCP2 assay showed the best diagnosis sensitivity (82%) and specificity (98%) with high positive predictive (PPV) (96%) and negative predictive values (NPV) (91%). Thirty percent of RA patients were carrying at least one copy of the HLA-DRB1 shared epitope (SE) compared to 10% and 14% of patients with other inflammatory rheumatic diseases and healthy individuals, respectively. The presence of the SE was associated with the production of ACPA. CONCLUSIONS: Anti-CCP2 antibodies are useful markers of RA in African patients. In this cohort, the prevalence of the SE is higher in RA patients than in controls but lower than that reported in patient cohorts of European ancestry. The discrepancy between the high prevalence of ACPA-positive patients and the relatively low number of SE-positive cases suggest that, in addition to SE, other genetic factors control the development of ACPA in African RA patients.
Keywords AdolescentAdultAfrican Continental Ancestry Group/ethnology/*geneticsAgedArthritis, Rheumatoid/*diagnosis/ethnology/*geneticsBiological Markers/bloodCameroon/epidemiologyEpitopes/genetics/immunologyFemaleHLA-DR Antigens/blood/*geneticsHLA-DRB1 ChainsHumansMaleMiddle AgedPeptides, Cyclic/*blood/immunologyPredictive Value of TestsYoung Adult
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PMID: 20196860
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Research groups Mécanisme de l'inflammation articulaire (44)
Compatibilité tissulaire et transplantation clinique (562)
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SINGWE-NGANDEU, Madeleine et al. Diagnostic value of anti-cyclic citrullinated peptides and association with HLA-DRB1 shared epitope alleles in African rheumatoid arthritis patients. In: Arthritis Research and Therapy, 2010, vol. 12, n° 2, p. R36. https://archive-ouverte.unige.ch/unige:20662

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Deposited on : 2012-05-22

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