Scientific article
Open access

Resistance of Dictyostelium discoideum membranes to saponin permeabilization

Published inBMC research notes, vol. 3, 120
Publication date2010

BACKGROUND: Saponin is a mild detergent commonly used to permeabilize cells prior to immunofluorescence labeling of intracellular proteins. It has previously been used to that effect in Dictyostelium discoideum amoebae. FINDINGS: We show that saponin, contrary to Triton X-100 or alcohol, permeabilizes at best incompletely membranes of Dictyostelium. In cells exposed to osmotic stress, almost complete resistance to saponin permeabilization was observed. CONCLUSIONS: Saponin should be used with special care to permeabilize Dictyostelium membranes. This unsusual property is presumably linked to the specific sterol composition of Dictyostelium membranes. It may also represent an adaptation of Dictyostelium to harsh conditions or to natural compounds encountered in its natural environment.

Research group
Citation (ISO format)
MERCANTI, Valentina, COSSON, Pierre. Resistance of Dictyostelium discoideum membranes to saponin permeabilization. In: BMC research notes, 2010, vol. 3, p. 120. doi: 10.1186/1756-0500-3-120
Main files (1)
Article (Accepted version)
ISSN of the journal1756-0500

Technical informations

Creation05/22/2012 3:16:07 PM
First validation05/22/2012 3:16:07 PM
Update time03/14/2023 5:30:49 PM
Status update03/14/2023 5:30:49 PM
Last indexation01/15/2024 11:40:58 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack