UNIGE document Scientific Article
previous document  unige:20652  next document
add to browser collection

Resistance of Dictyostelium discoideum membranes to saponin permeabilization

Published in BMC Research Notes. 2010, vol. 3, p. 120
Abstract BACKGROUND: Saponin is a mild detergent commonly used to permeabilize cells prior to immunofluorescence labeling of intracellular proteins. It has previously been used to that effect in Dictyostelium discoideum amoebae. FINDINGS: We show that saponin, contrary to Triton X-100 or alcohol, permeabilizes at best incompletely membranes of Dictyostelium. In cells exposed to osmotic stress, almost complete resistance to saponin permeabilization was observed. CONCLUSIONS: Saponin should be used with special care to permeabilize Dictyostelium membranes. This unsusual property is presumably linked to the specific sterol composition of Dictyostelium membranes. It may also represent an adaptation of Dictyostelium to harsh conditions or to natural compounds encountered in its natural environment.
PMID: 20426861
Full text
Research group Phagocytose (140)
(ISO format)
MERCANTI, Valentina, COSSON, Pierre. Resistance of Dictyostelium discoideum membranes to saponin permeabilization. In: BMC Research Notes, 2010, vol. 3, p. 120. https://archive-ouverte.unige.ch/unige:20652

262 hits



Deposited on : 2012-05-22

Export document
Format :
Citation style :