A casein kinase 1 and PAR proteins regulate asymmetry of a PIP(2) synthesis enzyme for asymmetric spindle positioning

Panbianco, Costanza; Weinkove, David; Zanin, Esther; Jones, David; Divecha, Nullin; Gotta, Monica; Ahringer, Julie

doi:10.1016/j.devcel.2008.06.002

Scientific article

English

A casein kinase 1 and PAR proteins regulate asymmetry of a PIP(2) synthesis enzyme for asymmetric spindle positioning

Contributors

Panbianco, Costanza; Weinkove, David; Zanin, Esther; Jones, David; Divecha, Nullin; Gotta, Monica

; Ahringer, Julie

Published in

Developmental cell, vol. 15, no. 2, p. 198-208

Publication date

2008

Abstract

Spindle positioning is an essential feature of asymmetric cell division. The conserved PAR proteins together with heterotrimeric G proteins control spindle positioning in animal cells, but how these are linked is not known. In C. elegans, PAR protein activity leads to asymmetric spindle placement through cortical asymmetry of Galpha regulators GPR-1/2. Here, we establish that the casein kinase 1 gamma CSNK-1 and a PIP(2) synthesis enzyme (PPK-1) transduce PAR polarity to asymmetric Galpha regulation. PPK-1 is posteriorly enriched in the one-celled embryo through PAR and CSNK-1 activities. Loss of CSNK-1 causes uniformly high PPK-1 levels, high symmetric cortical levels of GPR-1/2 and LIN-5, and increased spindle pulling forces. In contrast, knockdown of ppk-1 leads to low GPR-1/2 levels and decreased spindle forces. Furthermore, loss of CSNK-1 leads to increased levels of PIP(2). We propose that asymmetric generation of PIP(2) by PPK-1 directs the posterior enrichment of GPR-1/2 and LIN-5, leading to posterior spindle displacement.

Keywords

Animals
Caenorhabditis elegans/cytology/embryology/enzymology
Caenorhabditis elegans Proteins/metabolism
Casein Kinase I/metabolism
Cell Nucleus/enzymology
Embryo, Nonmammalian/cytology/enzymology
Mitotic Spindle Apparatus/enzymology
Models, Biological
Phosphatidylinositol 4,5-Diphosphate/metabolism
Phosphotransferases (Alcohol Group Acceptor)/metabolism
Protein Transport
Protein-Serine-Threonine Kinases/metabolism
RNA Interference

UNIGE affiliation entities

Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement

UNIGE research groups

La division asymétrique (847)

Citation (ISO format)

PANBIANCO, Costanza et al. A casein kinase 1 and PAR proteins regulate asymmetry of a PIP(2) synthesis enzyme for asymmetric spindle positioning. In: Developmental cell, 2008, vol. 15, n° 2, p. 198–208. doi: 10.1016/j.devcel.2008.06.002

Article (Accepted version)

Supplemental data - I

Supplemental data - II

Identifiers

PID : unige:2064
DOI : 10.1016/j.devcel.2008.06.002
PMID : 18694560

Additional URL for this publication

http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=18694560

Journal ISSN

1534-5807

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180downloads

Creation

17/06/2009 08:53:00

First validation

17/06/2009 08:53:00

Update

26/03/2026 10:36:01

Status update

26/03/2026 10:36:01

Last indexation

26/03/2026 10:41:04

A casein kinase 1 and PAR proteins regulate asymmetry of a PIP(2) synthesis enzyme for asymmetric spindle positioning

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

Main files (1)

Secondary files (2)

Identifiers

Additional URL for this publication

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation