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Title

A casein kinase 1 and PAR proteins regulate asymmetry of a PIP(2) synthesis enzyme for asymmetric spindle positioning

Authors
Weinkove, David
Zanin, Esther
Jones, David
Divecha, Nullin
Ahringer, Julie
Published in Developmental cell. 2008, vol. 15, no. 2, p. 198-208
Abstract Spindle positioning is an essential feature of asymmetric cell division. The conserved PAR proteins together with heterotrimeric G proteins control spindle positioning in animal cells, but how these are linked is not known. In C. elegans, PAR protein activity leads to asymmetric spindle placement through cortical asymmetry of Galpha regulators GPR-1/2. Here, we establish that the casein kinase 1 gamma CSNK-1 and a PIP(2) synthesis enzyme (PPK-1) transduce PAR polarity to asymmetric Galpha regulation. PPK-1 is posteriorly enriched in the one-celled embryo through PAR and CSNK-1 activities. Loss of CSNK-1 causes uniformly high PPK-1 levels, high symmetric cortical levels of GPR-1/2 and LIN-5, and increased spindle pulling forces. In contrast, knockdown of ppk-1 leads to low GPR-1/2 levels and decreased spindle forces. Furthermore, loss of CSNK-1 leads to increased levels of PIP(2). We propose that asymmetric generation of PIP(2) by PPK-1 directs the posterior enrichment of GPR-1/2 and LIN-5, leading to posterior spindle displacement.
Keywords AnimalsCaenorhabditis elegans/cytology/embryology/enzymologyCaenorhabditis elegans Proteins/metabolismCasein Kinase I/metabolismCell Nucleus/enzymologyEmbryo, Nonmammalian/cytology/enzymologyMitotic Spindle Apparatus/enzymologyModels, BiologicalPhosphatidylinositol 4,5-Diphosphate/metabolismPhosphotransferases (Alcohol Group Acceptor)/metabolismProtein TransportProtein-Serine-Threonine Kinases/metabolismRNA Interference
Identifiers
PMID: 18694560
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Other version: http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=18694560
Structures
Research group La division asymétrique (847)
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PANBIANCO, Costanza Viola Sofia et al. A casein kinase 1 and PAR proteins regulate asymmetry of a PIP(2) synthesis enzyme for asymmetric spindle positioning. In: Developmental cell, 2008, vol. 15, n° 2, p. 198-208. https://archive-ouverte.unige.ch/unige:2064

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Deposited on : 2009-06-17

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