Scientific article

A casein kinase 1 and PAR proteins regulate asymmetry of a PIP(2) synthesis enzyme for asymmetric spindle positioning

Published inDevelopmental cell, vol. 15, no. 2, p. 198-208
Publication date2008

Spindle positioning is an essential feature of asymmetric cell division. The conserved PAR proteins together with heterotrimeric G proteins control spindle positioning in animal cells, but how these are linked is not known. In C. elegans, PAR protein activity leads to asymmetric spindle placement through cortical asymmetry of Galpha regulators GPR-1/2. Here, we establish that the casein kinase 1 gamma CSNK-1 and a PIP(2) synthesis enzyme (PPK-1) transduce PAR polarity to asymmetric Galpha regulation. PPK-1 is posteriorly enriched in the one-celled embryo through PAR and CSNK-1 activities. Loss of CSNK-1 causes uniformly high PPK-1 levels, high symmetric cortical levels of GPR-1/2 and LIN-5, and increased spindle pulling forces. In contrast, knockdown of ppk-1 leads to low GPR-1/2 levels and decreased spindle forces. Furthermore, loss of CSNK-1 leads to increased levels of PIP(2). We propose that asymmetric generation of PIP(2) by PPK-1 directs the posterior enrichment of GPR-1/2 and LIN-5, leading to posterior spindle displacement.

  • Animals
  • Caenorhabditis elegans/cytology/embryology/enzymology
  • Caenorhabditis elegans Proteins/metabolism
  • Casein Kinase I/metabolism
  • Cell Nucleus/enzymology
  • Embryo, Nonmammalian/cytology/enzymology
  • Mitotic Spindle Apparatus/enzymology
  • Models, Biological
  • Phosphatidylinositol 4,5-Diphosphate/metabolism
  • Phosphotransferases (Alcohol Group Acceptor)/metabolism
  • Protein Transport
  • Protein-Serine-Threonine Kinases/metabolism
  • RNA Interference
Citation (ISO format)
PANBIANCO, Costanza et al. A casein kinase 1 and PAR proteins regulate asymmetry of a PIP(2) synthesis enzyme for asymmetric spindle positioning. In: Developmental cell, 2008, vol. 15, n° 2, p. 198–208. doi: 10.1016/j.devcel.2008.06.002
Main files (1)
Article (Accepted version)
Secondary files (2)
ISSN of the journal1878-1551

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