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Scientific article
Open access
English

Inhibition of Drp1-dependent mitochondrial fragmentation and apoptosis by a polypeptide antagonist of calcineurin

Published inCell death and differentiation, vol. 17, no. 11, p. 1785-1794
Publication date2010
Abstract

During apoptosis, mitochondria lose their membrane potential and undergo fragmentation around the time of release of cytochrome c. Apoptotic fission is at least in part sustained by the translocation of dynamin-related protein 1 (Drp1), normally located in the cytosol, to mitochondria. This process depends on dephosphorylation of Drp1 by the phosphatase calcineurin. Here, we report the identification of a novel inhibitor of this process. A polypeptide (PPD1) from the immunophilin FKBP52 inhibits calcineurin activation triggered by mitochondrial dysfunction. PPD1 blocks Drp1 translocation to mitochondria and fragmentation of the organelle. PPD1 delays apoptosis by intrinsic stimuli by preventing fragmentation and release of cytochrome c. Cells expressing PPD1 display enhanced clonogenic ability after exposure to staurosporine. A genetic analysis revealed that the activity of PPD1 is independent of the BH3-only protein BAD, another target of calcineurin during apoptosis, and is not additive to inhibition of Drp1. Thus, PPD1 is a novel inhibitor of apoptosis that elucidates the function of calcineurin-dependent mitochondrial fragmentation in the amplification of cell death.

Keywords
  • *Apoptosis/drug effects
  • Calcineurin/*antagonists & inhibitors/metabolism
  • Colony-Forming Units Assay
  • Cyclophilin A/metabolism
  • Cytochromes c/metabolism
  • Dyneins/metabolism
  • GTP Phosphohydrolases/antagonists & inhibitors/*metabolism
  • HeLa Cells
  • Humans
  • Membrane Potential, Mitochondrial/drug effects/physiology
  • Microtubule-Associated Proteins/antagonists & inhibitors/*metabolism
  • Mitochondria/drug effects/*metabolism/ultrastructure
  • Mitochondrial Membranes/metabolism
  • Mitochondrial Proteins/antagonists & inhibitors/*metabolism
  • Peptides/metabolism/*pharmacology
  • Phosphorylation
  • Protein Transport
  • Staurosporine/pharmacology
  • Tacrolimus Binding Proteins/metabolism/*pharmacology
  • Bcl-Associated Death Protein/metabolism
Citation (ISO format)
CEREGHETTI, G. M., COSTA, Veronica, SCORRANO, Luca. Inhibition of Drp1-dependent mitochondrial fragmentation and apoptosis by a polypeptide antagonist of calcineurin. In: Cell death and differentiation, 2010, vol. 17, n° 11, p. 1785–1794. doi: 10.1038/cdd.2010.61
Main files (1)
Article (Accepted version)
accessLevelPublic
Identifiers
ISSN of the journal1350-9047
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431downloads

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