Inhibition of Drp1-dependent mitochondrial fragmentation and apoptosis by a polypeptide antagonist of calcineurin

Cereghetti, G. M.; Costa, Veronica; Scorrano, Luca

doi:10.1038/cdd.2010.61

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Title		Inhibition of Drp1-dependent mitochondrial fragmentation and apoptosis by a polypeptide antagonist of calcineurin
Authors		Cereghetti, G. M. Costa, Veronica Scorrano, Luca
Published in		Cell Death and Differentiation. 2010, vol. 17, no. 11, p. 1785-1794
Abstract		During apoptosis, mitochondria lose their membrane potential and undergo fragmentation around the time of release of cytochrome c. Apoptotic fission is at least in part sustained by the translocation of dynamin-related protein 1 (Drp1), normally located in the cytosol, to mitochondria. This process depends on dephosphorylation of Drp1 by the phosphatase calcineurin. Here, we report the identification of a novel inhibitor of this process. A polypeptide (PPD1) from the immunophilin FKBP52 inhibits calcineurin activation triggered by mitochondrial dysfunction. PPD1 blocks Drp1 translocation to mitochondria and fragmentation of the organelle. PPD1 delays apoptosis by intrinsic stimuli by preventing fragmentation and release of cytochrome c. Cells expressing PPD1 display enhanced clonogenic ability after exposure to staurosporine. A genetic analysis revealed that the activity of PPD1 is independent of the BH3-only protein BAD, another target of calcineurin during apoptosis, and is not additive to inhibition of Drp1. Thus, PPD1 is a novel inhibitor of apoptosis that elucidates the function of calcineurin-dependent mitochondrial fragmentation in the amplification of cell death.
Keywords		Apoptosis/drug effects — Calcineurin/antagonists & inhibitors/metabolism — Colony-Forming Units Assay — Cyclophilin A/metabolism — Cytochromes c/metabolism — Dyneins/metabolism — GTP Phosphohydrolases/antagonists & inhibitors/metabolism — HeLa Cells — Humans — Membrane Potential, Mitochondrial/drug effects/physiology — Microtubule-Associated Proteins/antagonists & inhibitors/metabolism — Mitochondria/drug effects/metabolism/ultrastructure — Mitochondrial Membranes/metabolism — Mitochondrial Proteins/antagonists & inhibitors/metabolism — Peptides/metabolism/pharmacology — Phosphorylation — Protein Transport — Staurosporine/pharmacology — Tacrolimus Binding Proteins/metabolism/pharmacology — Bcl-Associated Death Protein/metabolism
Identifiers		DOI: 10.1038/cdd.2010.61 PMID: 20489733
Full text		Article (Accepted version) - Free access Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3000862/pdf/ukmss-33560.pdf
Structures		Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme
Research group		Les mitochondries dans la vie cellulaire (850)
Citation (ISO format)		CEREGHETTI, G. M., COSTA, Veronica, SCORRANO, Luca. Inhibition of Drp1-dependent mitochondrial fragmentation and apoptosis by a polypeptide antagonist of calcineurin. In: Cell Death and Differentiation, 2010, vol. 17, n° 11, p. 1785-1794. doi: 10.1038/cdd.2010.61 https://archive-ouverte.unige.ch/unige:20616