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Intestinal lamina propria TcRgammadelta+ lymphocytes selectively express IL-10 and IL-17

Asigbetse, K. E.
Frossard, C. P.
Published in Journal of Investigational Allergology & Clinical Immunology. 2010, vol. 20, no. 5, p. 391-401
Abstract BACKGROUND: The characteristics and roles of gut lymphocytes have been only partly elucidated, in particular with regard to activation patterns. OBJECTIVES: To characterize lymphocytes from various parts of the gut and examine their activation pattern as a network. METHODS: Lymphocytes were isolated from the epithelium, the lamina propria, Peyer's patches, mesenteric lymph nodes, the spleen, and peripheral blood of naive mice. They were then characterized for T cell phenotype, T cell receptors (TcRs), activation markers, and cytokine production. RESULTS: The results showed a gradient of cells with an increasing proportion of TcRgammadelta+, CD8alphaalpha+ cells towards the gut lumen, with the highest number found in intraepithelial lymphocytes. These cells, together with lamina propria lymphocytes (LPLs) were also characterized by a memory-like phenotype (CD25- CD45RB(low) and CD44(high)) and CD69 expression. CD8+ TcRgammadelta+ LPLs produced IL-10 and IL-17, while TcRalphabeta+ LPLs were FoxP3 positive. CONCLUSIONS: Gut lymphocytes express various receptors and cytokines according to their location. These specific features suggest a differential function for gut lymphocytes depending on their location.
Keywords AnimalsAntigens, CD/biosynthesisFemaleForkhead Transcription Factors/biosynthesisImmunologic MemoryImmunophenotypingInterleukin-10/*secretionInterleukin-17/*secretionIntestinal Mucosa/cytology/*immunology/metabolismLymphocyte ActivationMiceMice, Inbred C3HReceptors, Antigen, T-Cell, gamma-delta/*metabolismT-Lymphocytes/cytology/immunology/*metabolism
PMID: 20945605
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Research group Pathogenèse et traitement des allergies alimentaires (191)
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ASIGBETSE, K. E., EIGENMANN, Philippe, FROSSARD, C. P. Intestinal lamina propria TcRgammadelta+ lymphocytes selectively express IL-10 and IL-17. In: Journal of Investigational Allergology and Clinical Immunology, 2010, vol. 20, n° 5, p. 391-401. https://archive-ouverte.unige.ch/unige:20429

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Deposited on : 2012-05-22

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