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Titles listAssessment of topical bioavailability in vivo: the importance of stratum corneum thickness
Titles listAssessment of topical bioavailability in vivo: the importance of stratum corneum thickness
Scientific Article
unige:20365 
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Assessment of topical bioavailability in vivo: the importance of stratum corneum thickness |
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| Authors | ||
| Published in | Skin Pharmacology and Applied Skin Physiology. 2001, vol. 14 Suppl 1, p. 82-6 | |
| Abstract | We present a method to determine the cutaneous bioavailability and hence to evaluate the bioequivalence of topically applied drugs in vivo. The procedure uses serial tape-stripping and transepidermal water loss measurements to quantify the thickness of the removed stratum corneum (SC) and to determine the intact membrane thickness. Following tape-stripping, the drug is extracted from the tapes and assayed, e.g., by HPLC. This provides a drug concentration profile as a function of the normalized position within the SC. The data are fitted to a solution of Fick's second law of diffusion in order to calculate characteristic membrane transport parameters. Integration of the concentration profile over the entire SC thickness, that is, the 'area-under-the-curve', provides a measure of the cutaneous bioavailability and hence can be used to assess the bioequivalence of topically applied drugs. | |
| Keywords | Administration, Topical — Animals — Biological Availability — Humans — Skin/anatomy & histology — Skin Absorption/physiology | |
| Identifiers | DOI: 10.1159/000056396 PMID: 11509911 | |
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| Citation (ISO format) | KALIA, Yogeshvar et al. Assessment of topical bioavailability in vivo: the importance of stratum corneum thickness. In: Skin Pharmacology and Applied Skin Physiology, 2001, vol. 14 Suppl 1, p. 82-6. https://archive-ouverte.unige.ch/unige:20365 |
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