en
Scientific article
English

Assessment of topical bioavailability in vivo: the importance of stratum corneum thickness

Published inSkin pharmacology and applied skin physiology, vol. 14 Suppl 1, p. 82-86
Publication date2001
Abstract

We present a method to determine the cutaneous bioavailability and hence to evaluate the bioequivalence of topically applied drugs in vivo. The procedure uses serial tape-stripping and transepidermal water loss measurements to quantify the thickness of the removed stratum corneum (SC) and to determine the intact membrane thickness. Following tape-stripping, the drug is extracted from the tapes and assayed, e.g., by HPLC. This provides a drug concentration profile as a function of the normalized position within the SC. The data are fitted to a solution of Fick's second law of diffusion in order to calculate characteristic membrane transport parameters. Integration of the concentration profile over the entire SC thickness, that is, the 'area-under-the-curve', provides a measure of the cutaneous bioavailability and hence can be used to assess the bioequivalence of topically applied drugs.

Keywords
  • Administration, Topical
  • Animals
  • Biological Availability
  • Humans
  • Skin/anatomy & histology
  • Skin Absorption/physiology
Citation (ISO format)
KALIA, Yogeshvar et al. Assessment of topical bioavailability in vivo: the importance of stratum corneum thickness. In: Skin pharmacology and applied skin physiology, 2001, vol. 14 Suppl 1, p. 82–86. doi: 10.1159/000056396
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal1422-2868
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