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Molecular imaging by micro-CT: specific E-selectin imaging

Wyss, Caroline
Schaefer, Stephan C.
Juillerat-Jeanneret, Lucienne
Lagopoulos, Lucienne
Lehr, Hans-Anton
Published in European Radiology. 2009, vol. 19, no. 10, p. 2487-2494
Collection Open Access - Licence nationale Springer
Abstract The primary goal of this study was to design a fluorescent E-selectin-targeted iodine-containing liposome for specific E-selectin imaging with the use of micro-CT. The secondary goal was to correlate the results of micro-CT imaging with other imaging techniques with cellular resolution, i.e., confocal and intravital microscopy. E-selectin-targeted liposomes were tested on endothelial cells in culture and in vivo in HT-29 tumor-bearing mice (n = 12). The liposomes contained iodine (as micro-CT contrast medium) and fluorophore (as optical contrast medium) for confocal and intravital microscopy. Optical imaging methods were used to confirm at the cellular level, the observations made with micro-CT. An ischemia-reperfusion model was used to trigger neovessel formation for intravital imaging. The E-selectin-targeted liposomes were avidly taken up by activated endothelial cells, whereas nontargeted liposomes were not. Direct binding of the E-selectin-targeted liposomes was proved by intravital microscopy, where bright spots clearly appeared on the activated vessels. Micro-CT imaging also demonstrated accumulation of the targeted lipsomes into subcutaneous tumor by an increase of 32 + or - 8 HU. Hence, internalization by activated endothelial cells was rapid and mediated by E-selectin. We conclude that micro-CT associated with specific molecular contrast agent is able to detect specific molecular markers on activated vessel walls in vivo.
Keywords AnimalsContrast MediaE-Selectin/*diagnostic use/*metabolismHT29 CellsHumansMiceMice, Inbred C57BLMolecular Imaging/*methodsNeovascularization, Pathologic/*metabolism/*radiographyTomography, X-Ray Computed/*methodsTumor Markers, Biological/*analysis
PMID: 19440717
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Research group Imagerie et radiologie interventionnelle du foie, des voies biliaires et du pancréas (316)
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WYSS, Caroline et al. Molecular imaging by micro-CT: specific E-selectin imaging. In: European Radiology, 2009, vol. 19, n° 10, p. 2487-2494. https://archive-ouverte.unige.ch/unige:20182

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Deposited on : 2012-04-23

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