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Title

Impact of a short period of pre-enrichment on detection and bacterial loads of methicillin-resistant Staphylococcus aureus from screening specimens

Authors
Van Heirstraeten, L.
Cortinas Abrahantes, J.
Lammens, C.
Lee, A.
Molenberghs, G.
Aerts, M.
Goossens, H.
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Published in Journal of Clinical Microbiology. 2009, vol. 47, no. 10, p. 3326-3328
Abstract We compared the impacts of direct plating on a chromogenic medium and of plating after enrichment (4 h and overnight) on the detection of methicillin-resistant Staphylococcus aureus (MRSA) from 52 patient screening samples. MRSA colony counts for approximately 70% of samples after overnight pre-enrichment were >20-fold higher than the counts after the other two treatments. The qualitative differences (sample was MRSA positive/negative) between the results of the direct plating and 4-h pre-enrichment treatments were not significant, indicating no advantage of the latter; however, the number of samples positive for MRSA increased significantly after an overnight sample pre-enrichment (P < 0.005).
Keywords Bacteriological Techniques/*methodsColony Count, MicrobialHumansMass Screening/*methodsMethicillin-Resistant Staphylococcus aureus/*isolation & purificationSensitivity and SpecificityStaphylococcal Infections/*diagnosis
Identifiers
PMID: 19675216
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Research group Groupe Harbarth Stephan (Staphylocoques dorés résistants à la méthicilline) (866)
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VAN HEIRSTRAETEN, L. et al. Impact of a short period of pre-enrichment on detection and bacterial loads of methicillin-resistant Staphylococcus aureus from screening specimens. In: Journal of Clinical Microbiology, 2009, vol. 47, n° 10, p. 3326-3328. https://archive-ouverte.unige.ch/unige:20147

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Deposited on : 2012-04-23

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