Efficient IgM assembly and secretion require the plasma cell induced endoplasmic reticulum protein pERp1

van Anken, Eelco; Pena, Florentina; Hafkemeijer, Nicole; Christis, Chantal; Romijn, Edwin P.; Grauschopf, Ulla; Oorschot, Viola M. J.; Pertel, Thomas; Engels, Sander; Ora, Ari; Lastun, Viorica; Glockshuber, Rudi; Klumperman, Judith; Heck, Albert J. R.; Luban, Jeremy; Braakman, Ineke

doi:10.1073/pnas.0903036106

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Title		Efficient IgM assembly and secretion require the plasma cell induced endoplasmic reticulum protein pERp1
Authors		van Anken, Eelco Pena, Florentina Hafkemeijer, Nicole Christis, Chantal Romijn, Edwin P. Grauschopf, Ulla Oorschot, Viola M. J. Pertel, Thomas Engels, Sander Ora, Ari Lastun, Viorica Glockshuber, Rudi Klumperman, Judith Heck, Albert J. R. Luban, Jeremy Braakman, Ineke show all authors [1 - 16]
Published in		Proceedings of the National Academy of Sciences. 2009, vol. 106, no. 40, p. 17019-17024
Abstract		Plasma cells daily secrete their own mass in antibodies, which fold and assemble in the endoplasmic reticulum (ER). To reach these levels, cells require pERp1, a novel lymphocyte-specific small ER-resident protein, which attains expression levels as high as BiP when B cells differentiate into plasma cells. Although pERp1 has no homology with known ER proteins, it does contain a CXXC motif typical for oxidoreductases. In steady state, the CXXC cysteines are locked by two parallel disulfide bonds with a downstream C(X)(6)C motif, and pERp1 displays only modest oxidoreductase activity. pERp1 emerged as a dedicated folding factor for IgM, associating with both heavy and light chains and promoting assembly and secretion of mature IgM.
Keywords		Amino Acid Sequence — Animals — B-Lymphocytes/metabolism/ultrastructure — Cell Differentiation — Cell Line, Tumor — Electrophoresis, Gel, Two-Dimensional — Endoplasmic Reticulum/metabolism — HeLa Cells — Heat-Shock Proteins/genetics/metabolism — Humans — Immunoblotting — Immunoglobulin M/metabolism — Mass Spectrometry — Mice — Microscopy, Fluorescence — Microscopy, Immunoelectron — Molecular Chaperones/genetics/metabolism — Oxidoreductases/metabolism — Plasma Cells/cytology/metabolism — RNA Interference — Sulfhydryl Compounds/metabolism
Identifiers		DOI: 10.1073/pnas.0903036106 PMID: 19805154
Full text		Article - Limited access to UNIGE Other version: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2761347/pdf/zpq17019.pdf
Structures		Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire
Research group		Réplication virale, pathogénèse et immunité (848)
Citation (ISO format)		VAN ANKEN, Eelco et al. Efficient IgM assembly and secretion require the plasma cell induced endoplasmic reticulum protein pERp1. In: Proceedings of the National Academy of Sciences, 2009, vol. 106, n° 40, p. 17019-17024. doi: 10.1073/pnas.0903036106 https://archive-ouverte.unige.ch/unige:20145