en
Scientific article
English

Efficient IgM assembly and secretion require the plasma cell induced endoplasmic reticulum protein pERp1

Publication date2009
Abstract

Plasma cells daily secrete their own mass in antibodies, which fold and assemble in the endoplasmic reticulum (ER). To reach these levels, cells require pERp1, a novel lymphocyte-specific small ER-resident protein, which attains expression levels as high as BiP when B cells differentiate into plasma cells. Although pERp1 has no homology with known ER proteins, it does contain a CXXC motif typical for oxidoreductases. In steady state, the CXXC cysteines are locked by two parallel disulfide bonds with a downstream C(X)(6)C motif, and pERp1 displays only modest oxidoreductase activity. pERp1 emerged as a dedicated folding factor for IgM, associating with both heavy and light chains and promoting assembly and secretion of mature IgM.

Keywords
  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes/metabolism/ultrastructure
  • Cell Differentiation
  • Cell Line, Tumor
  • Electrophoresis, Gel, Two-Dimensional
  • Endoplasmic Reticulum/*metabolism
  • HeLa Cells
  • Heat-Shock Proteins/genetics/metabolism
  • Humans
  • Immunoblotting
  • Immunoglobulin M/*metabolism
  • Mass Spectrometry
  • Mice
  • Microscopy, Fluorescence
  • Microscopy, Immunoelectron
  • Molecular Chaperones/genetics/*metabolism
  • Oxidoreductases/metabolism
  • Plasma Cells/cytology/*metabolism
  • RNA Interference
  • Sulfhydryl Compounds/metabolism
Citation (ISO format)
VAN ANKEN, Eelco et al. Efficient IgM assembly and secretion require the plasma cell induced endoplasmic reticulum protein pERp1. In: Proceedings of the National Academy of Sciences of the United States of America, 2009, vol. 106, n° 40, p. 17019–17024. doi: 10.1073/pnas.0903036106
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Identifiers
ISSN of the journal0027-8424
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