UNIGE document Scientific Article
previous document  unige:20037  next document
add to browser collection
Title

Improved cutaneous healing in diabetic mice exposed to healthy peripheral circulation

Authors
Scherer, Sandra S.
Alperovich, Michael
Chen, Bin
Orgill, Dennis P.
Wagers, Amy J.
Published in Journal of Investigative Dermatology. 2009, vol. 129, no. 9, p. 2265-2274
Abstract Impaired repair of skin defects is a major complication of diabetes; yet, the pathophysiology of diabetic (db) wound healing remains largely opaque. Here, we investigate the role of humoral factors in modulating db wound repair by generating chimeric animals through parabiotic joining of wild-type (wt) and diabetic (db/db) mice. This strategy allows wounds on healing-deficient db/db mice to be exposed to factors derived from the wt circulation at physiologically appropriate concentrations. When compared with db controls, chimeric db/db animals showed significantly improved healing of full-thickness, cutaneous wounds, with enhanced granulation tissue formation, angiogenesis, cell proliferation, and collagen deposition. Glycemic control was unaffected by parabiosis; however, the distribution of circulating leukocytes, altered in db controls, normalized in db-chimeras. Both wt and db cells were recruited from circulation into db wounds, but wt cells never exceeded 20% of total cells. Improved angiogenesis persisted in db-chimeras separated 24 hours after wounding, suggesting the existence of long-term normalizing factors. This study establishes a new model for studying db wound healing, and shows a key role for circulating factors in normalizing wound repair in diabetes.
Keywords AnimalsCell ProliferationCollagen/metabolismDiabetes Mellitus/blood/*physiopathologyFemaleGranulation Tissue/physiopathologyMiceMice, Inbred C57BLNeovascularization, Physiologic*ParabiosisWound Healing/*physiology
Identifiers
PMID: 19295612
Full text
Structures
Research group Groupe Pittet Cuenod Brigitte (chirurgie plastique et reconstructive) (96)
Citation
(ISO format)
PIETRAMAGGIORI, Giorgio et al. Improved cutaneous healing in diabetic mice exposed to healthy peripheral circulation. In: Journal of Investigative Dermatology, 2009, vol. 129, n° 9, p. 2265-2274. https://archive-ouverte.unige.ch/unige:20037

203 hits

0 download

Update

Deposited on : 2012-04-23

Export document
Format :
Citation style :