en
Scientific article
English

Assessment of hepatic steatosis by expert pathologists: the end of a gold standard

Published inAnnals of surgery, vol. 250, no. 5, p. 691-697
Publication date2009
Abstract

BACKGROUND: The presence of fat in the liver is considered a major risk for postoperative complication after liver surgery and transplantation. The current standard of quantification of hepatic steatosis is microscopic evaluation by pathologists, although consistency in such assessment remains unclear. Computerized image analysis is an alternative method for objective assessment of the degree of hepatic steatosis. METHODS: High resolution images of hematoxylin and eosin stained liver sections from 46 consecutive patients, initially diagnosed with liver steatosis, were blindly assessed by 4 established expert pathologists from different institutions. Computerized analysis was carried out simultaneously on the same sections. Interobserver agreement and correlation between the pathologists' and computerized assessment were evaluated using intraclass correlation coefficients (ICC), Spearman rank correlation coefficients, or descriptive statistics. RESULTS: Poor agreement among pathologists (ICC: 0.57) was found regarding the assessment of total steatosis, (ICC >0.7 indicates acceptable agreement). Pathologists' estimation of micro- and macrosteatosis disclosed also poor correlation (ICC: 0.22, 0.55, respectively). Inconsistent assessment of histological features of steatohepatitis (lobular inflammation, portal inflammation, hepatocyte ballooning, and Mallory hyaline) was documented. Poor conformity was also shown between the computerized quantification and ratings of 3 pathologists (Spearman rank correlation coefficients: 0.22, 0.82, 0.28, and 0.38). CONCLUSION: Quantification of hepatic steatosis in histological sections is strongly observer-dependent, not reproducible, and does not correlate with the computerized estimation. Current standards of assessment, previously published data and the clinical relevance of hepatic steatosis for liver surgery and transplantation must be challenged.

Keywords
  • Adult
  • Aged
  • Aged, 80 and over
  • Coloring Agents
  • Diagnosis, Computer-Assisted
  • Fatty Liver/*pathology
  • Female
  • Hepatocytes/metabolism/pathology
  • Histological Techniques
  • Humans
  • Image Processing, Computer-Assisted
  • Lipids/analysis
  • Liver/*pathology
  • Male
  • Middle Aged
  • Observer Variation
  • Pathology, Clinical
Citation (ISO format)
EL-BADRY, Ashraf Mohammad et al. Assessment of hepatic steatosis by expert pathologists: the end of a gold standard. In: Annals of surgery, 2009, vol. 250, n° 5, p. 691–697. doi: 10.1097/SLA.0b013e3181bcd6dd
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ISSN of the journal0003-4932
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