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Dictyostelium Tom1 participates to an ancestral ESCRT-0 complex

Mattei, Sara
Aubry, Laurence
Smith, Ewan W.
Letourneur, François
Published in Traffic. 2009, vol. 10, no. 2, p. 161-171
Abstract Sorting of ubiquitinated proteins to multivesicular bodies (MVBs) in mammalian cells relies on proteins with a Vps27/Hrs/STAM (VHS) domain. Here, we show that the amoeba Dictyostelium presents only one protein with a VHS domain: DdTom1. We demonstrate that the VHS domain of DdTom1 is followed by a Golgi-localized, gamma-ear-containing, ADP-ribosylation-factor-binding and Tom1 (GAT) domain that binds ubiquitin, and by a non-conserved C-terminal domain that can recruit clathrin, EGFr pathway substrate 15 and tumor susceptibility gene 101, a component of the MVB biogenesis machinery [endosomal complexes required for transport (ESCRT) complexes]. Both VHS and GAT domains interact with phospholipids and therefore could ensure the recruitment of DdTom1 to endosomal membranes. We propose that DdTom1 participates in an ancestral ESCRT-0 complex implicated in the sorting of ubiquitinated proteins into MVBs.
Keywords Adaptor Proteins, Vesicular Transport/metabolismAmino Acid MotifsAnimalsCell-Derived Microparticles/*metabolismClathrin/metabolismCytoplasm/metabolismDictyostelium/genetics/*metabolism/ultrastructureGene DeletionHumansLysosomes/metabolismMicroscopy, ElectronProtein BindingProtozoan Proteins/genetics/*metabolism
PMID: 19054384
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Research group Phagocytose (140)
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BLANC, Cédric et al. Dictyostelium Tom1 participates to an ancestral ESCRT-0 complex. In: Traffic, 2009, vol. 10, n° 2, p. 161-171. doi: 10.1111/j.1600-0854.2008.00855.x https://archive-ouverte.unige.ch/unige:19685

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Deposited on : 2012-04-23

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