Improved survival of fulminant liver failure by transplantation of microencapsulated cryopreserved porcine hepatocytes in mice

Mei, Jie; Sgroi, Antonino; Mai, Gang; Baertschiger, Reto Marc; Gonelle-Gispert, Carmen; Serre-Beinier, Veronique; Morel, Philippe; Buehler, Leo Hans

doi:10.3727/096368909788237168

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Title		Improved survival of fulminant liver failure by transplantation of microencapsulated cryopreserved porcine hepatocytes in mice
Authors		Mei, Jie Sgroi, Antonino Mai, Gang Baertschiger, Reto Marc Gonelle-Gispert, Carmen Serre-Beinier, Veronique Morel, Philippe Buehler, Leo Hans
Published in		Cell Transplantation. 2009, vol. 18, no. 1, p. 101-110
Abstract		The aim of this study was to establish hepatocyte isolation in pigs, and to evaluate function of isolated hepatocytes after encapsulation, cryopreservation, and transplantation (Tx) in a mouse model of fulminant liver failure (FLF). After isolation, porcine hepatocytes were microencapsulated with alginate-poly-L-Lysine-alginate membranes and cryopreserved. In vitro, albumin production of free and encapsulated hepatocytes were measured by enzyme linked-immunoadsorbent assay. In vivo, encapsulated hepatocytes were transplanted into different groups of mice with FLF and the following experimental groups were performed: group 1, Tx of empty capsules; group 2, Tx of free primary porcine hepatocytes; group 3, Tx of fresh encapsulated porcine hepatocytes; group 4, Tx of cryopreserved encapsulated porcine hepatocytes. In vitro, fresh or cryopreserved encapsulated porcine hepatocytes showed a continuous decreasing metabolic function over 1 week (albumin and urea synthesis, drug catabolism). In vivo, groups 1 and 2 showed similar survival (18% and 25%, respectively, p > 0.05). In groups 3 and 4, Tx of fresh or cryopreserved encapsulated porcine hepatocytes significantly increased survival rate to 75% and 68%, respectively (p < 0.05). Primary porcine hepatocytes maintained metabolic functions after encapsulation and cryopreservation. In mice with FLF, Tx of encapsulated xenogeneic hepatocytes significantly improved survival. These results indicate that porcine hepatocytes can successfully be isolated, encapsulated, stored using cryopreservation, and transplanted into xenogeneic recipients with liver failure and sustain liver metabolic functions.
Keywords		Animals — Cells, Cultured — Cryopreservation/methods — Drug Compounding — Graft Survival — Hepatocytes/metabolism/transplantation — Liver Failure, Acute/metabolism/therapy — Male — Mice — Mice, Inbred C57BL — Microscopy, Electron, Transmission — Swine — Transplantation, Heterologous/methods
Identifiers		DOI: 10.3727/096368909788237168 PMID: 19476213
Full text		Article (Published version) (4.1 MB) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine clinique / Département de chirurgie
Research groups		Chirurgie viscérale (104) Chirurgie viscérale (HUG) Cultures cellulaires et transplantations (519)
Citation (ISO format)		MEI, Jie et al. Improved survival of fulminant liver failure by transplantation of microencapsulated cryopreserved porcine hepatocytes in mice. In: Cell Transplantation, 2009, vol. 18, n° 1, p. 101-110. doi: 10.3727/096368909788237168 https://archive-ouverte.unige.ch/unige:19558