UNIGE document Scientific Article
previous document  unige:19527  next document
add to browser collection
Title

Synovial tissues concentrate secreted APRIL

Authors
Krenn, Veit
Published in Arthritis Research and Therapy. 2009, vol. 11, no. 5, p. R144
Abstract INTRODUCTION: A proliferation-inducing ligand (APRIL) from the TNF family, owing to its role in the generation and survival of plasma cells (PCs), is currently targeted for rheumatoid arthritis (RA) treatment. However, little is known about APRIL expression in RA lesions, hampering our understanding of the way APRIL may modulate this autoimmune disease. METHODS: We performed immunological staining of human normal, non-RA and RA synovial tissues with a pair of antibodies specifically recognizing APRIL-producing cells and secreted APRIL. RESULTS: We detected significant amounts of secreted APRIL in normal synovium mostly concentrated around blood vessels and at the lining layer, but no cells producing APRIL. Meanwhile, we observed that blood neutrophils constitutively secrete APRIL, indicating that blood APRIL may diffuse into the synovium via its fenestrated vessels. Synovium from non-RA and RA patients retained similarly secreted APRIL, but in this case APRIL-producing cells, including neutrophils and macrophages, were present in the tissue. Notably, PCs--when present in RA synovium--accumulated in areas of APRIL retention, spreading from blood vessels towards the lining layer. CONCLUSIONS: PCs accumulate in synovial zones rich in secreted APRIL, consistent with a pro-survival role of APRIL for PCs in RA. The concentration of APRIL by normal synovium indicates that this tissue may constitute a proper environment for PCs even before RA onset.
Keywords AdultAgedAged, 80 and overArthritis, Rheumatoid/*metabolismFemaleFlow CytometryHumansImmunohistochemistryMaleMiddle AgedNeutrophils/metabolismPlasma Cells/*metabolismSynovial Membrane/*secretionTumor Necrosis Factor Ligand Superfamily Member 13/*secretion
Identifiers
PMID: 19788740
Full text
Structures
Research groups Mécanisme de l'inflammation articulaire (44)
La matrice extracellulaire (651)
Citation
(ISO format)
GABAY, Cem et al. Synovial tissues concentrate secreted APRIL. In: Arthritis Research and Therapy, 2009, vol. 11, n° 5, p. R144. https://archive-ouverte.unige.ch/unige:19527

228 hits

206 downloads

Update

Deposited on : 2012-04-23

Export document
Format :
Citation style :