Mass fractionation of <i>Drosophila</i> egg chambers

Jacobs-Lorena, Marcelo; Crippa, Marco

doi:10.1016/0012-1606(77)90223-8

Scientific article

English

Mass fractionation of Drosophila egg chambers

ContributorsJacobs-Lorena, Marcelo; Crippa, Marco

Published inDevelopmental biology, vol. 57, no. 2, p. 385-392

Publication date1977-06

Abstract

Egg chambers from Drosophila ovaries may now be fractionated in large quantities suitable for biochemical work. A suspension of isolated egg chambers is prepared by subjecting ovaries to digestion with 0.067% collagenase. Egg chambers are separated in size classes (which correspond to different developmental stages) by sieving them through a column of superimposed nylon nets. The method can, in principle, be used for the separation of any particles ranging upward from 10 μm in diameter.

Keywords

Animals
Cell Separation / methods
Drosophila melanogaster / cytology
Female
Microbial Collagenase / metabolism
Oogenesis
Ovary / cytology

NoteTravaux du Département de biologie animale ; vol. 23, no 696

UNIGE affiliation entities

Faculté des sciences / Section de biologie / Département de génétique et évolution

Citation (ISO format)

JACOBS-LORENA, Marcelo, CRIPPA, Marco. Mass fractionation of Drosophila egg chambers. In: Developmental biology, 1977, vol. 57, n° 2, p. 385–392. doi: 10.1016/0012-1606(77)90223-8

Article (Published version)

Identifiers

PID : unige:195070
DOI : 10.1016/0012-1606(77)90223-8
PMID : 194805

Journal ISSN0012-1606

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Mass fractionation of Drosophila egg chambers

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