Scientific article

Ubp3 requires a cofactor, Bre5, to specifically de-ubiquitinate the COPII protein, Sec23

Published inNature cell biology, vol. 5, no. 7, p. 661-667
Publication date2003

Ubiquitination is important for a broad array of cellular functions. Although reversal of this process, de-ubiquitination, most probably represents an important regulatory step contributing to cellular homeostasis, the specificity and properties of de-ubiquitination enzymes remain poorly understood. Here, we show that the Saccharomyces cerevisiae ubiquitin protease Ubp3 requires an additional protein, Bre5, to form an active de-ubiquitination complex that cleaves ubiquitin from specific substrates. In particular, this complex rescues Sec23p, a COPII subunit essential for the transport between the endoplasmic reticulum and the Golgi apparatus, from degradation by the proteasome. This probably contributes to maintaining and adapting a Sec23 expression level that is compatible with an efficient secretion pathway, and consequently with cell growth and viability.

  • COP-Coated Vesicles/metabolism/ultrastructure
  • Caenorhabditis elegans Proteins
  • Carrier Proteins/genetics/metabolism
  • Cells, Cultured
  • Cysteine Endopeptidases/metabolism/ultrastructure
  • Endopeptidases/deficiency/genetics/metabolism
  • Endoplasmic Reticulum/metabolism/ultrastructure
  • GTPase-Activating Proteins
  • Galactosyltransferases/deficiency/genetics
  • Golgi Apparatus/metabolism/ultrastructure
  • Microscopy, Electron
  • Multienzyme Complexes/metabolism/ultrastructure
  • Proteasome Endopeptidase Complex
  • Protein Transport/physiology
  • Saccharomyces cerevisiae/metabolism/ultrastructure
  • Saccharomyces cerevisiae Proteins/genetics/metabolism
  • Ubiquitin/metabolism
  • Ubiquitin Thiolesterase
Citation (ISO format)
COHEN, Mickaël et al. Ubp3 requires a cofactor, Bre5, to specifically de-ubiquitinate the COPII protein, Sec23. In: Nature cell biology, 2003, vol. 5, n° 7, p. 661–667. doi: 10.1038/ncb1003
Main files (1)
Article (Published version)
ISSN of the journal1465-7392

Technical informations

Creation04/13/2012 10:23:00 AM
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