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Title

Ubiquitin-associated domain of Mex67 synchronizes recruitment of the mRNA export machinery with transcription

Authors
Gwizdek, Carole
Rodriguez, Manuel S
Ossareh-Nazari, Batool
Hobeika, Maria
Divita, Gilles
Dargemont, Catherine
Published in Proceedings of the National Academy of Sciences. 2006, vol. 103, no. 44, p. 16376-81
Abstract The mRNA nuclear export receptor Mex67/Mtr2 is recruited to mRNAs through RNA-binding adaptors, including components of the THO/TREX complex that couple transcription to mRNA export. Here we show that the ubiquitin-associated (UBA) domain of Mex67 is not only required for proper nuclear export of mRNA but also contributes to recruitment of Mex67 to transcribing genes. Our results reveal that the UBA domain of Mex67 directly interacts with polyubiquitin chains and with Hpr1, a component of the THO/TREX complex, which is regulated by ubiquitylation in a transcription-dependent manner. This interaction transiently protects Hpr1 from ubiquitin/proteasome-mediated degradation and thereby coordinates recruitment of the mRNA export machinery with transcription and early messenger ribonucleoproteins assembly.
Keywords Biological TransportDNA-Binding Proteins/metabolismNuclear Proteins/genetics/metabolismNucleocytoplasmic Transport Proteins/genetics/metabolismProteasome Endopeptidase Complex/metabolismProtein BindingRNA, Messenger/geneticsRNA-Binding Proteins/genetics/metabolismSaccharomyces cerevisiae/genetics/metabolismSaccharomyces cerevisiae Proteins/genetics/metabolismTranscription, Genetic/geneticsUbiquitin/metabolism
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PMID: 17056718
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GWIZDEK, Carole et al. Ubiquitin-associated domain of Mex67 synchronizes recruitment of the mRNA export machinery with transcription. In: Proceedings of the National Academy of Sciences, 2006, vol. 103, n° 44, p. 16376-81. https://archive-ouverte.unige.ch/unige:19430

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Deposited on : 2012-04-17

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