UNIGE document Scientific Article
previous document  unige:19428  next document
add to browser collection

THO/Sub2p functions to coordinate 3'-end processing with gene-nuclear pore association

Rougemaille, Mathieu
Kisseleva-Romanova, Elena
Gudipati, Rajani Kanth
Lemoine, Sophie
Blugeon, Corinne
Boulay, Jocelyne
Jensen, Torben Heick
show hidden authors show all authors [1 - 11]
Published in Cell. 2008, vol. 135, no. 2, p. 308-21
Abstract During transcription, proteins assemble sequentially with nascent RNA to generate a messenger ribonucleoprotein particle (mRNP). The THO complex and its associated Sub2p helicase are functionally implicated in both transcription and mRNP biogenesis but their precise function remains elusive. We show here that THO/Sub2p mutation leads to the accumulation of a stalled intermediate in mRNP biogenesis that contains nuclear pore components and polyadenylation factors in association with chromatin. Microarray analyses of genomic loci that are aberrantly docked to the nuclear pore in mutants allowed the identification of approximately 400 novel validated target genes that require THO /Sub2p for efficient expression. Our data strongly suggests that the THO complex/Sub2p function is required to coordinate events leading to the acquisition of export competence at a step that follows commitment to 3'-processing.
Keywords Active Transport, Cell NucleusAdenosine Triphosphatases/genetics/metabolismChromatin/metabolismHeat-Shock Proteins/geneticsMutationNuclear Pore/metabolismNuclear Proteins/metabolismNucleocytoplasmic Transport Proteins/metabolismNucleosomes/metabolismRNA 3' End ProcessingRNA Polymerase II/metabolismRNA TransportRNA, Fungal/metabolismRNA-Binding Proteins/metabolismRibonucleoproteins/metabolismSaccharomyces cerevisiae/metabolismSaccharomyces cerevisiae Proteins/genetics/metabolismTranscription, Genetic
PMID: 18957205
Full text
Article (Published version) (1.1 MB) - document accessible for UNIGE members only Limited access to UNIGE
(ISO format)
ROUGEMAILLE, Mathieu et al. THO/Sub2p functions to coordinate 3'-end processing with gene-nuclear pore association. In: Cell, 2008, vol. 135, n° 2, p. 308-21. doi: 10.1016/j.cell.2008.08.005 https://archive-ouverte.unige.ch/unige:19428

465 hits

0 download


Deposited on : 2012-04-17

Export document
Format :
Citation style :