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Title

THO/Sub2p functions to coordinate 3'-end processing with gene-nuclear pore association

Authors
Rougemaille, Mathieu
Kisseleva-Romanova, Elena
Gudipati, Rajani Kanth
Lemoine, Sophie
Blugeon, Corinne
Boulay, Jocelyne
Jensen, Torben Heick
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Published in Cell. 2008, vol. 135, no. 2, p. 308-21
Abstract During transcription, proteins assemble sequentially with nascent RNA to generate a messenger ribonucleoprotein particle (mRNP). The THO complex and its associated Sub2p helicase are functionally implicated in both transcription and mRNP biogenesis but their precise function remains elusive. We show here that THO/Sub2p mutation leads to the accumulation of a stalled intermediate in mRNP biogenesis that contains nuclear pore components and polyadenylation factors in association with chromatin. Microarray analyses of genomic loci that are aberrantly docked to the nuclear pore in mutants allowed the identification of approximately 400 novel validated target genes that require THO /Sub2p for efficient expression. Our data strongly suggests that the THO complex/Sub2p function is required to coordinate events leading to the acquisition of export competence at a step that follows commitment to 3'-processing.
Keywords Active Transport, Cell NucleusAdenosine Triphosphatases/genetics/metabolismChromatin/metabolismHeat-Shock Proteins/geneticsMutationNuclear Pore/metabolismNuclear Proteins/metabolismNucleocytoplasmic Transport Proteins/metabolismNucleosomes/metabolismRNA 3' End ProcessingRNA Polymerase II/metabolismRNA TransportRNA, Fungal/metabolismRNA-Binding Proteins/metabolismRibonucleoproteins/metabolismSaccharomyces cerevisiae/metabolismSaccharomyces cerevisiae Proteins/genetics/metabolismTranscription, Genetic
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PMID: 18957205
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ROUGEMAILLE, Mathieu et al. THO/Sub2p functions to coordinate 3'-end processing with gene-nuclear pore association. In: Cell, 2008, vol. 135, n° 2, p. 308-21. https://archive-ouverte.unige.ch/unige:19428

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Deposited on : 2012-04-17

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