UNIGE document Doctoral Thesis
previous document  unige:1936  next document
add to browser collection
Title

The transcription elongation factor P-TEFb in 'Drosophila melanogaster' : characterization of a mutation of the Cdk9 kinase and of the different activities of two co-factors

Author
Directors
Defense Thèse de doctorat : Univ. Genève, 2009 - Sc. 4080 - 2009/04/06
Abstract Transcription by RNA polymerase II is regulated, in part, by the positive transcription elongation factor b (P-TEFb), which promotes transition from abortive to productive elongation. The Drosophila P-TEFb complex is composed of the Cdk9 kinase and a cyclin partner, CyclinT or CyclinK. To investigate the physiological role of P-TEFb, we generated transgenic flies allowing the conditional expression of wild-type or mutant Cdk9, alone or together with CyclinT or CyclinK. We found that the two P-TEFb complexes have similar binding pattern on chromosomes and are recruited to transcriptionally active loci. By expressing a dominant-negative form of Cdk9 in specific tissues, we showed that P-TEFb function is required for endoreplication of larval tissues, for proper differentiation of imaginal discs and for oogenesis. We demonstrated that the two cyclin subunits have non-redundant activities in vivo, and that P-TEFb containing CyclinT, but not CyclinK, activates transcription when tethered to promoter.
Keywords TranscriptionP-TEFbRNA Polymerase IICdk9Cycline
Identifiers
URN: urn:nbn:ch:unige-19367
Full text
Thesis (5.7 MB) - public document Free access
Structures
Citation
(ISO format)
BASQUIN, Denis. The transcription elongation factor P-TEFb in 'Drosophila melanogaster' : characterization of a mutation of the Cdk9 kinase and of the different activities of two co-factors. Université de Genève. Thèse, 2009. https://archive-ouverte.unige.ch/unige:1936

251 hits

556 downloads

Update

Deposited on : 2009-06-10

Export document
Format :
Citation style :