The transcription elongation factor P-TEFb in &#039;Drosophila melanogaster&#039; : characterization of a mutation of the Cdk9 kinase and of the different activities of two co-factors

Basquin, Denis

doi:10.13097/archive-ouverte/unige:1936

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Title		The transcription elongation factor P-TEFb in 'Drosophila melanogaster' : characterization of a mutation of the Cdk9 kinase and of the different activities of two co-factors
Author		Basquin, Denis
Directors		Spierer, Pierre Pauli, Daniel
Defense		Thèse de doctorat : Univ. Genève, 2009 - Sc. 4080 - 2009/04/06
Abstract		Transcription by RNA polymerase II is regulated, in part, by the positive transcription elongation factor b (P-TEFb), which promotes transition from abortive to productive elongation. The Drosophila P-TEFb complex is composed of the Cdk9 kinase and a cyclin partner, CyclinT or CyclinK. To investigate the physiological role of P-TEFb, we generated transgenic flies allowing the conditional expression of wild-type or mutant Cdk9, alone or together with CyclinT or CyclinK. We found that the two P-TEFb complexes have similar binding pattern on chromosomes and are recruited to transcriptionally active loci. By expressing a dominant-negative form of Cdk9 in specific tissues, we showed that P-TEFb function is required for endoreplication of larval tissues, for proper differentiation of imaginal discs and for oogenesis. We demonstrated that the two cyclin subunits have non-redundant activities in vivo, and that P-TEFb containing CyclinT, but not CyclinK, activates transcription when tethered to promoter.
Keywords		Transcription — P-TEFb — RNA Polymerase II — Cdk9 — Cycline
Identifiers		DOI: 10.13097/archive-ouverte/unige:1936 URN: urn:nbn:ch:unige-19367
Full text		Thesis (5.7 MB) - Free access
Structures		Faculté des sciences / Section de biologie / Département de génétique et évolution
Citation (ISO format)		BASQUIN, Denis. The transcription elongation factor P-TEFb in 'Drosophila melanogaster' : characterization of a mutation of the Cdk9 kinase and of the different activities of two co-factors. Université de Genève. Thèse, 2009. doi: 10.13097/archive-ouverte/unige:1936 https://archive-ouverte.unige.ch/unige:1936

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Deposited on : 2009-06-10

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The transcription elongation factor P-TEFb in 'Drosophila melanogaster' : characterization of a mutation of the Cdk9 kinase and of the different activities of two co-factors