Scientific article
English

Negative feedback regulation of UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis

Publication date2010
Abstract

Plants respond to low levels of UV-B radiation with a coordinated photomorphogenic response that allows acclimation to this environmental stress factor. The key players in this UV-B response are COP1 (an E3 ubiquitin ligase), UVR8 (a β-propeller protein), and HY5 (a bZIP transcription factor). We have shown previously that an elevated UV-B-specific response is associated with dwarf growth, indicating the importance of balancing UV-B-specific signaling. Negative regulators of this pathway are not known, however. Here, we describe two highly related WD40-repeat proteins, REPRESSOR OF UV-B PHOTOMORPHOGENESIS 1 (RUP1) and RUP2, that interact directly with UVR8 as potent repressors of UV-B signaling. Both genes were transcriptionally activated by UV-B in a COP1-, UVR8-, and HY5-dependent manner. rup1 rup2 double mutants showed an enhanced response to UV-B and elevated UV-B tolerance after acclimation. Overexpression of RUP2 resulted in reduced UV-B-induced photomorphogenesis and impaired acclimation, leading to hypersensitivity to UV-B stress. These results are consistent with an important regulatory role for RUP1 and RUP2, which act downstream of UVR8-COP1 in a negative feedback loop impinging on UVR8 function, balancing UV-B defense measures and plant growth.

Keywords
  • Acclimatization/radiation effects
  • Arabidopsis/growth & development/radiation effects
  • Arabidopsis Proteins/genetics/metabolism
  • Feedback, Physiological/radiation effects
  • Gene Expression Regulation, Plant/radiation effects
  • Morphogenesis/radiation effects
  • Mutation/genetics
  • Protein Binding/radiation effects
  • RNA, Messenger/genetics/metabolism
  • Signal Transduction/radiation effects
  • Stress, Physiological/radiation effects
  • Ultraviolet Rays
Citation (ISO format)
GRUBER, Henriette et al. Negative feedback regulation of UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis. In: Proceedings of the National Academy of Sciences of the United States of America, 2010, vol. 107, n° 46, p. 20132–20137. doi: 10.1073/pnas.0914532107
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0027-8424
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