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Granzymes and cell death

Thiery, Jerome
Chowdhury, Dipanjan
Published in Methods in Enzymology. 2008, vol. 442, p. 213-230
Abstract Granzymes are cell death-inducing serine proteases released from cytotoxic granules of cytotoxic T lymphocytes and natural killer cells during granule exocytosis in response to viral infection or against transformed cells marked for elimination. A critical cofactor for the granule exocytosis pathway is perforin, which mediates the entry of granzymes into target cells, where they cleave specific substrates that initiate DNA fragmentation and apoptosis. One of the biggest challenges in studying the biology of granzymes has been the functional redundancy of granzymes in animal models making an in vitro experimental system essential. This chapter discusses methods to study granzyme function in vitro under physiologically relevant experimental conditions.
Keywords AnimalsApoptosis/*physiologyBaculoviridae/geneticsCell Death/physiologyCell LineCell Line, TumorEscherichia coli/geneticsGranzymes/genetics/isolation & purification/*metabolismMicroscopy, FluorescencePerforin/metabolismRatsRecombinant Proteins/isolation & purification/metabolismSpodopteraSubstrate Specificity
PMID: 18662572
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Research group ROS in CTL mediated cell death : from mechanism to applications (898)
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MARTINVALET, Denis, THIERY, Jerome, CHOWDHURY, Dipanjan. Granzymes and cell death. In: Methods in Enzymology, 2008, vol. 442, p. 213-230. doi: 10.1016/S0076-6879(08)01411-0 https://archive-ouverte.unige.ch/unige:19171

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Deposited on : 2012-03-27

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