Reactive oxygen species and fatigue-induced prolonged low-frequency force depression in skeletal muscle fibres of rats, mice and SOD2 overexpressing mice

Bruton, Joseph D.; Place, Nicolas; Yamada, Takashi; Silva, Jose P.; Andrade, Francisco H.; Dahlstedt, Anders J.; Zhang, Shi-Jin; Katz, Abram; Larsson, Nils-Goran; Westerblad, Hakan

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Title		Reactive oxygen species and fatigue-induced prolonged low-frequency force depression in skeletal muscle fibres of rats, mice and SOD2 overexpressing mice
Authors		Bruton, Joseph D. Place, Nicolas Yamada, Takashi Silva, Jose P. Andrade, Francisco H. Dahlstedt, Anders J. Zhang, Shi-Jin Katz, Abram Larsson, Nils-Goran Westerblad, Hakan show all authors [1 - 10]
Published in		The Journal of Physiology. 2008, vol. 586, no. 1, p. 175-184
Abstract		Skeletal muscle often shows a delayed force recovery after fatiguing stimulation, especially at low stimulation frequencies. In this study we focus on the role of reactive oxygen species (ROS) in this fatigue-induced prolonged low-frequency force depression. Intact, single muscle fibres were dissected from flexor digitorum brevis (FDB) muscles of rats and wild-type and superoxide dismutase 2 (SOD2) overexpressing mice. Force and myoplasmic free [Ca(2+)] ([Ca(2+)](i)) were measured. Fibres were stimulated at different frequencies before and 30 min after fatigue induced by repeated tetani. The results show a marked force decrease at low stimulation frequencies 30 min after fatiguing stimulation in all fibres. This decrease was associated with reduced tetanic [Ca(2+)](i) in wild-type mouse fibres, whereas rat fibres and mouse SOD2 overexpressing fibres instead displayed a decreased myofibrillar Ca(2+) sensitivity. The SOD activity was approximately 50% lower in wild-type mouse than in rat FDB muscles. Myoplasmic ROS increased during repeated tetanic stimulation in rat fibres but not in wild-type mouse fibres. The decreased Ca(2+) sensitivity in rat fibres could be partially reversed by application of the reducing agent dithiothreitol, whereas the decrease in tetanic [Ca(2+)](i) in wild-type mouse fibres was not affected by dithiothreitol or the antioxidant N-acetylcysteine. In conclusion, we describe two different causes of fatigue-induced prolonged low-frequency force depression, which correlate to differences in SOD activity and ROS metabolism. These findings may have clinical implications since ROS-mediated impairments in myofibrillar function can be counteracted by reductants and antioxidants, whereas changes in SR Ca(2+) handling appear more resistant to interventions.
Keywords		Acetylcysteine/pharmacology — Animals — Antioxidants/pharmacology — Calcium/metabolism — Dithiothreitol/pharmacology — Gene Expression Regulation, Enzymologic — Male — Mice — Mice, Inbred Strains — Muscle Contraction/drug effects/physiology — Muscle Fatigue/physiology — Muscle, Skeletal/metabolism — Rats — Reactive Oxygen Species/metabolism — Sarcoplasmic Reticulum/metabolism — Superoxide Dismutase/genetics/*metabolism
Identifiers		DOI: 10.1113/jphysiol.2007.147470 PMID: 18006575
Full text		Article - Limited access to UNIGE Other version: http://jp.physoc.org/content/586/1/175.full.pdf
Structures		Faculté de médecine / Ecole d'éducation physique et de sport
Citation (ISO format)		BRUTON, Joseph D. et al. Reactive oxygen species and fatigue-induced prolonged low-frequency force depression in skeletal muscle fibres of rats, mice and SOD2 overexpressing mice. In: The Journal of Physiology, 2008, vol. 586, n° 1, p. 175-184. https://archive-ouverte.unige.ch/unige:19155