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Scientific article
English

A dual task for the Xbp1-responsive OS-9 variants in the mammalian endoplasmic reticulum: inhibiting secretion of misfolded protein conformers and enhancing their disposal

Published inThe Journal of biological chemistry, vol. 283, no. 24, p. 16446-16454
Publication date2008
Abstract

Normally, non-native polypeptides are not transported through the secretory pathway. Rather, they are translocated from the endoplasmic reticulum (ER) lumen into the cytosol where they are degraded by proteasomes. Here we characterize the function in ER quality control of two proteins derived from alternative splicing of the OS-9 gene. OS-9.1 and OS-9.2 are ubiquitously expressed in human tissues and are amplified in tumors. They are transcriptionally induced upon activation of the Ire1/Xbp1 ER-stress pathway. OS-9 variants do not associate with folding-competent proteins. Rather, they selectively bind folding-defective ones thereby inhibiting transport of non-native conformers through the secretory pathway. The intralumenal level of OS-9.1 and OS-9.2 inversely correlates with the fraction of a folding-defective glycoprotein, the Null(hong kong) (NHK) variant of alpha1-antitrypsin that escapes retention-based ER quality control. OS-9 up-regulation does not affect NHK disposal, but reduction of the intralumenal level of OS-9.1 and OS-9.2 substantially delays disposal of this model substrate. OS-9.1 and OS-9.2 also associate transiently with non-glycosylated folding-defective proteins, but association is unproductive. Finally, OS-9 activity does not require an intact mannose 6-P homology domain. Thus, OS-9.1 and OS-9.2 play a dual role in mammalian ER quality control: first as crucial retention factors for misfolded conformers, and second as promoters of protein disposal from the ER lumen.

Keywords
  • Cell Line
  • Cell Membrane/metabolism
  • DNA-Binding Proteins/*metabolism
  • Endoplasmic Reticulum/*metabolism
  • Glycoproteins/metabolism
  • Humans
  • Lectins
  • Lentivirus/metabolism
  • Models, Biological
  • Neoplasm Proteins/*genetics/metabolism
  • Nuclear Proteins/*metabolism
  • Proteasome Endopeptidase Complex/metabolism
  • Protein Conformation
  • Protein Structure, Tertiary
  • Subcellular Fractions/metabolism
  • Transcription Factors
  • Transcriptional Activation
  • Up-Regulation
Citation (ISO format)
BERNASCONI, Riccardo et al. A dual task for the Xbp1-responsive OS-9 variants in the mammalian endoplasmic reticulum: inhibiting secretion of misfolded protein conformers and enhancing their disposal. In: The Journal of biological chemistry, 2008, vol. 283, n° 24, p. 16446–16454. doi: 10.1074/jbc.M802272200
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ISSN of the journal0021-9258
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