Scientific article

Association of TSC1/hamartin, 14-3-3sigma, and p27 expression with tumor outcomes in patients with pTa/pT1 urothelial bladder carcinoma

Published inAmerican journal of clinical pathology, vol. 129, no. 6, p. 918-923
Publication date2008

TSC1/hamartin is a tumor suppressor gene involved in the development of various malignancies, including bladder cancer. In vitro studies showed that hamartin controls cell proliferation partly by up-regulating p27 and 14-3-3sigma. This study was designed to explore the value of these biomarkers in predicting outcome in pTa/pT1 tumors and validate the regulation of p27 and 14-3-3sigma by hamartin in vivo using human bladder cancer tissue. A tissue microarray of 134 pTa and pT1 tumors was constructed, and sections were stained with hamartin, 14-3-3sigma, and p27 antibodies. In multiple Cox regression analysis, pTa/pT1 tumors with reduced or low expression of hamartin tended to have higher risk of progression (P = .030). High-grade tumors tended to be at higher risk of progression in comparison with low-grade tumors (P < .001). The combination of expression of these 3 biomarkers did not add predictive value regarding disease outcomes. Low hamartin expression and high tumor grade are independent factors in predicting faster pTa/pT1 tumor progression. In a subset of pTa/pT1 tumors, hamartin has a role in bladder carcinogenesis by positively regulating 14-3-3sigma and p27.

  • 14-3-3 Proteins
  • Adult
  • Aged
  • Aged, 80 and over
  • Carcinoma, Transitional Cell/*genetics/metabolism/pathology
  • Cyclin-Dependent Kinase Inhibitor p27/*genetics/metabolism
  • DNA, Neoplasm/analysis
  • Exonucleases/*genetics/metabolism
  • Female
  • *Gene Expression Regulation, Neoplastic
  • Humans
  • Male
  • Middle Aged
  • Neoplasm Proteins/*genetics/metabolism
  • Neoplasm Recurrence, Local
  • Retrospective Studies
  • Tissue Array Analysis
  • Tumor Markers, Biological/*genetics/metabolism
  • Tumor Suppressor Proteins/*genetics/metabolism
  • Urinary Bladder Neoplasms/*genetics/metabolism/pathology
  • Urothelium/metabolism/pathology
Citation (ISO format)
MHAWECH-FAUCEGLIA, Paulette et al. Association of TSC1/hamartin, 14-3-3sigma, and p27 expression with tumor outcomes in patients with pTa/pT1 urothelial bladder carcinoma. In: American journal of clinical pathology, 2008, vol. 129, n° 6, p. 918–923. doi: 10.1309/D81QMXPMC3QHT57Y
Updates (1)
ISSN of the journal0002-9173

Technical informations

Creation03/27/2012 9:23:03 AM
First validation03/27/2012 9:23:03 AM
Update time03/14/2023 5:19:52 PM
Status update03/14/2023 5:19:52 PM
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