The SWI/SNF complex is an essential nucleosome remodeler, which generates chromatin accessibility to control gene expression. Mutations in SWI/SNF subunits are frequent in various neurodevelopmental disorders and cancers. In this thesis, we aimed to increase our mechanistic understanding of SWI/SNF regulation and identify novel therapeutic targets for treating diseases caused by SWI/SNF mutations. First, we developed a live/dead reporter of SWI/SNF activity in mouse embryonic stem cells and performed a genome-wide CRISPR KO screen to uncover novel modulators of SWI/SNF function. Followingly, we validated three hits and uncovered the molecular mechanisms linking each novel modulator to SWI/SNF activity. First, we discovered that the m⁶A-writer subunit RBM15 controls stoichiometric assembly of SWI/SNF complexes. Next, we discovered that the MLF2 protein acts as a chaperone of SWI/SNF complex assembly. Finally, we found that the chromatin modifier PHF6 and SWI/SNF coregulate the expression of key neurodevelopmental genes. Overall, this study gives valuable insights into different modes of SWI/SNF regulation.