Book chapter
English

The Use of Grating-Coupled Interferometry to Study Inositol Pyrophosphate–Protein Interactions

Published inSaiardi, A., Fiedler, D., Jessen, H.J. (Ed.), Inositol Pyrophosphates - Methods and Protocols, p. 153-169
PublisherNew York : Humana Press
Collection
  • Methods in Molecular Biology; 2972
Publication date2025
First online date2025-08-29
Abstract

Grating-coupled interferometry (GCI) is a biophysical method suitable to studying the binding kinetics of protein-ligand interactions. Here, we describe the application of biotinylated inositol pyrophosphate (PP-InsP) nutrient messengers to characterize PP-InsP-protein interactions using a Creoptix WAVEsystem.

Keywords
  • Binding kinetics
  • Creoptix WAVE
  • Grating-coupled interferometry
  • Inositol pyrophosphates
  • Protein-ligand interaction
Research groups
Citation (ISO format)
STURM, Kristina, HOTHORN, Michael. The Use of Grating-Coupled Interferometry to Study Inositol Pyrophosphate–Protein Interactions. In: Inositol Pyrophosphates - Methods and Protocols. Saiardi, A., Fiedler, D., Jessen, H.J. (Ed.). New York : Humana Press, 2025. p. 153–169. (Methods in Molecular Biology) doi: 10.1007/978-1-0716-4799-8_12
Main files (1)
Book chapter (Published version)
accessLevelRestricted
Identifiers
ISBN978-1-0716-4799-8
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Technical informations

Creation01/10/2025 14:00:41
First validation06/10/2025 09:17:28
Update06/10/2025 09:17:28
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