The Passa Três granite is a 5 km2 intrusion in southern Brazil with an NNE–SSW-elongated shape, hosting gold-bearing quartz veins with fluorite, carbonates, sulphides (pyrite, chalcopyrite, aikinite, molybdenite) and native gold. Orebodies are hosted by the pluton roof zone, which is marked by various textures indicating magmatic–hydrothermal transition processes. Mineralisation formed between 613 and 608 Ma in extensional pull-apart structures controlled by two conjugated N–S and E–W fault systems. We report results from petrography, quantitative evaluation of minerals by scanning electron microscopy (QEMSCAN), scanning electron microscopy (SEM), electron probe microanalyses (EPMA), X-ray fluorescence (XRF), trace element analyses of pyrite by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), sulphur isotope (δ34S) analyses, and fluid inclusion microthermometry. Hydrothermal alteration is dominantly developed as phengite–quartz–carbonate and sericite–carbonate–chlorite assemblages along mineralised structures. Fluid inclusion study indicates mineralising fluids with H2O-CO2-NaCl composition, low to moderate salinity (0.2–12.84 wt % NaCl eq.), and temperatures from 400 to 150 °C. The sulphur isotopic composition of pyrite (−0.1 to 1.1‰) suggests magmatic origin. These data, in conjunction with structurally controlled mineralisation enriched in Au-Bi minerals shortly post-dating the granite emplacement, appoint towards similarities between the Passa Três deposit and intrusion-related gold systems. The specific location of the mineralisation in the core (and in the roof zone, regarding its vertical position) of the Passa Três granitic intrusion defines it as “granite-hosted” thus, it is representative of a specific model that can be used for exploration of other intrusion-related/hosted gold deposits near the studied area or in other locations.