Scientific article

Toll-like receptor-7 agonist decoration enhances the adjuvanticity of chitosan-DNA nanoparticles

Published inJournal of pharmaceutical sciences, vol. 101, no. 3, p. 1166-1177
Publication date2012

In order to provide an adjuvant-equipped carrier system for plasmid deoxyribonucleic acid (pDNA) vaccines, we grafted for the first time a Toll-like receptor (TLR)-7 agonistic moiety [9-benzyl-8-hydroxyadenine (HA)] through a poly(ethylene glycol) (PEG) spacer onto a water-soluble chitosan derivative [final copolymer: 6-0-carboxymethyl-N,N,N-trimethylchitosan (CTC)-graft-PEG-HA (CTCPHA)]. Successful grafting was confirmed by spectroscopic (H NMR, mass, ultraviolet-visible, and Fourier transform infrared spectroscopy) and chromatographic (size-exclusion chromatography-multi-angle laser light scattering) methods. In this article, TLR-7 agonist-decorated CTCPHA nanoparticles (NPs) were formulated by complex coacervation with pDNA expressing the green fluorescence protein. Resulting NPs had a size of around 200 nm with a positive surface charge and high DNA encapsulation efficiency. In contrast to the use of DNA alone, NP protected DNA against enzymatic degradation and enabled transfection of alveolar A549 cells. Interestingly, TLR-7 agonist decoration increased significantly the interleukin-8-related immune stimulatory capacity of polymeric chitosan and chitosan-based NP in human THP-1 macrophages when compared with controls. In summary, we demonstrate here the proof-of-principle that covalent TLR-7 agonist functionalization of chitosan-DNA NPs enhances the carrier's adjuvanticity, representing a valuable concept for future polymer-based DNA vaccination.

  • Chitosan
  • Nanoparticles
  • Genetic vaccination
  • Nonviral gene delivery
  • Mucosalt vaccination
  • Vaccine adjuvants
  • Toll-like receptor agonist
  • THP-1 macrophages
  • IL-8 release
Citation (ISO format)
HEUKING, Simon, BORCHARD, Gerrit. Toll-like receptor-7 agonist decoration enhances the adjuvanticity of chitosan-DNA nanoparticles. In: Journal of pharmaceutical sciences, 2012, vol. 101, n° 3, p. 1166–1177. doi: 10.1002/jps.23017
Main files (1)
Article (Published version)
ISSN of the journal0022-3549

Technical informations

Creation02/28/2012 11:49:00 AM
First validation02/28/2012 11:49:00 AM
Update time03/14/2023 5:09:22 PM
Status update03/14/2023 5:09:21 PM
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