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Title

Pulmonary delivery of DNA encoding Mycobacterium tuberculosis latency antigen Rv1733c associated to PLGA–PEI nanoparticles enhances T cell responses in a DNA prime/protein boost vaccination regimen in mice
Authors
Bivas-Benita, Maytal
Lin, May Young
Bal, Suzanne M.
van Meijgaarden, Krista E.
Franken, Kees L.M.C.
Friggen, Annemieke H.
Junginger, Hans E.
Borchard, Gerrit
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Published in Vaccine. 2009, vol. 27, no. 30, p. 4010-4017
Abstract During persistent infection and hypoxic-stress, Mycobacterium tuberculosis (Mtb) expresses a series of Mtb latency antigens. The aim of this study was to evaluate the immunogenicity of a DNA vaccine encoding the Mtb latency antigen Rv1733c and to explore the effect of pulmonary delivery and co-formulation with poly (d,l-lactide-co-glycolide) (PLGA)-polyethyleneimine (PEI) nanoparticles (np) on host immunity. Characterization studies indicated that PLGA-PEI np kept their nanometer size after concentration and were positively charged. The np were able to mature human dendritic cells and stimulated them to secrete IL-12 and TNF-alpha comparable to levels observed after lipopolysaccharide (LPS) stimulation. Mtb latency antigen Rv1733c DNA prime combined with Rv1733c protein boost enhanced T cell proliferation and IFN-gamma secretion in mice in response to Rv1733c and Mtb hypoxic lysate. Rv1733c DNA adsorbed to PLGA-PEI np and applied to the lungs increased T cell proliferation and IFN-gamma production more potently compared to the same vaccinations given intramuscularly. The strongest immunogenicity was obtained by pulmonary priming with np-adsorbed Rv1733c DNA followed by boosting with Rv1733c protein. These results confirm that PLGA-PEI np are an efficient DNA vaccine delivery system to enhance T cell responses through pulmonary delivery in a DNA prime/protein boost vaccine regimen.
Keywords DNA vaccine — Tuberculosis latency antigens — Pulmonary immunization — PLGA-PEI nanoparticles — T cell response
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Other version: http://linkinghub.elsevier.com/retrieve/pii/S0264410X09005787
Structures
Faculté des sciences / Section des sciences pharmaceutiques
Citation
(ISO format) 		BIVAS-BENITA, Maytal et al. Pulmonary delivery of DNA encoding Mycobacterium tuberculosis latency antigen Rv1733c associated to PLGA–PEI nanoparticles enhances T cell responses in a DNA prime/protein boost vaccination regimen in mice. In: Vaccine, 2009, vol. 27, n° 30, p. 4010-4017. https://archive-ouverte.unige.ch/unige:18729

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Deposited on : 2012-03-06

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